Publications by authors named "Yu Fen Samantha Seah"
Microfluidic single-cell technology in immunology and antibody screening.
Mol Aspects Med
February 2018
Single-cell technology has a major impact on the field of immunology. It enables the kinetics and logic of immune signaling and immune cell migration to be elucidated, facilitates antibody screening and allows massively parallelized analysis of B- and T-cell repertoires. Impressive progress has been made over the last decade, strongly boosted by microfluidic approaches.
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- Embryonic stem cells (ESCs) can self-renew and differentiate into any cell type from the three germ layers, while somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs).
- Advances in gene editing techniques like CRISPR and TALENs have improved the efficiency of making precise changes in genomes.
- Combining somatic cell reprogramming with gene editing allows for better in vitro modeling of human diseases compared to traditional animal models, and the review highlights current strategies, advancements, and challenges in this area.
Embryonic stem cells (ESCs) repress the expression of exogenous proviruses and endogenous retroviruses (ERVs). Here, we systematically dissected the cellular factors involved in provirus repression in embryonic carcinomas (ECs) and ESCs by a genome-wide siRNA screen. Histone chaperones (Chaf1a/b), sumoylation factors (Sumo2/Ube2i/Sae1/Uba2/Senp6), and chromatin modifiers (Trim28/Eset/Atf7ip) are key determinants that establish provirus silencing.
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