Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty.

Mesenchymal stem cells from the adipose tissue of patients with postoperative hernias produce excessive amounts of collagen III, which shifts the balance between type III and type I collagens. The proposed technique of pretransplantation preparation allows in vitro stimulation collagen formation processes with predominant activation of collagen I synthesis and normalization of proportion between different collagen types. Abdominal wall repair with polypropylene surgical mesh in combination with autotransplantation of mesenchymal stem cells reduced the collagen III to collagen I ratio due to activation of collagen I synthesis and suppression of collagen III production, which had a positive effect on the structure of in vivo formed connective tissue.