Serotyping of Avibacterium paragallinarum isolates from Peru.

This study appears to represent the first serotyping study of 24 isolates of Avibacterium paragallinarum obtained from different regions of Peru during 1998-2008. All isolates were characterized as beta-nicotinamide adenine dinucleotide dependent. According to the Page scheme, modified by Blackall, it was found that eight isolates were classified as serogroup A, seven isolates as serogroup B, and five isolates as serogroup C, while four isolates could not be serotyped.

Amplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum.

A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying hens from different regions of Peru. The 16S rDNA was amplified by PCR using a pair of bacterial universal primers and restriction analysis of 16S rDNA sequences was done to select endonucleases with the highest number of cutting points inside the 16S rDNA. The DNA patterns with DdeI and RsaI endonucleases were identical for the 19 A.

Intraspecies genetic variability of Ornithobacterium rhinotracheale in commercial birds in Peru.

Strains of the bacterium Ornithobacterium rhinotracheale (ORT), a causal agent of respiratory diseases in birds, were microbiologically isolated, identified, and molecularly characterized. Blood-enriched culture media and biochemistry tests were used for microbiologic identification. Polymerase chain reaction (PCR) and repetitive extragenic palindromic PCR (rep-PCR) techniques were used for molecular identification and characterization, respectively, of the microorganism.