Methane seepage intensity distinguish microbial communities in sediments at the Mid-Okinawa Trough.

Shallow methane/sulfate transition zones in cold seeps are hotspots to study microbially mediated geochemical cycles due to high methane fluxes. However, our knowledge about the microbial communities in remote seafloor cold seep ecosystems with different methane seepage intensity is still sparse due to the challenge for sampling and visual observations. In this work, three remotely operated vehicle (ROV) video-guided push sediment cores were sampled from cold seep fields with different methane seepage intensity (low-intensity seepage, R5-C1; moderate-intensity seepage, R6-C2; high-intensity seepage, R6-C3) at the western slope of Mid-Okinawa Trough (Mid-OT) and subjected to high throughput sequencing of 16S rRNA genes for bacteria and archaea.

Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough.

Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic oxidation of methane (AOM), in sediments from an active cold seep in the mid-Okinawa Trough. Methane-oxidizing archaea, including ANME-1a, ANME-1b, ANME-2a/b, ANME-2c, and ANME-3, were detected in the OT cold seep sediments.

Suppression of attenuates adipogenesis with a downregulation of LXR-SREBP1 pathway in 3T3-L1 cells.

Previous RNA-Seq analyses revealed that NAD(P)H steroid dehydrogenase-like () has a different expression during 3T3-L1 differentiation; however, its roles in adipogenesis are unknown. In the present study, using quantitative real-time PCR, we confirmed that knockdown increased the proliferation of 3T3-L1 preadipocytes, but attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of gene expression. Further analyses showed that the expression peak of was at the early stage of 3T3-L1 preadipocytes differentiation and signaling pathway was downregulated in -knockdown 3T3-L1 cells.

Adipogenesis associated Mth938 domain containing (AAMDC) protein expression is regulated by alternative polyadenylation and microRNAs.

The post-transcriptional events regulating expression of the adipogenesis associated Mth938 domain containing (AAMDC) protein are poorly understood. Here, we find that AAMDC expresses three isoforms due to alternative polyadenylation (APA) and alternative splicing (AS). Luciferase assay revealed that expression of the two isoforms with short and long 3'UTRs is differentially controlled.

RNA-Seq analysis reveals a negative role of MSMO1 with a synergized NSDHL expression during adipogenesis of 3T3-L1.

To investigate a comprehensive transcriptome information of adipogenesis, we assessed global changes in the transcriptional events during 3T3-L1 adipogenesis by RNA-Seq. Compared to the preadipocyte stage (day 0), gene expression profiling demonstrated that 2013 genes were up-regulated, and 2430 genes were down-regulated at the differentiated adipocyte stage (day 13). Among these differentially expressed genes, we found the expression of MSMO1 was down-regulated at day 13, but whether it impacts adipogenesis has not been characterized.