The validation and cross-cultural adaptation of the PainDETECT questionnaire in osteoarthritis-related pain.

Background: Patients with knee osteoarthritis (KOA) often experience persistent pain and functional impairment after total knee arthroplasty (TKA), which presents challenges for pain management. Accurate preoperative assessment of pain characteristics is crucial for tailoring individualized treatment plans. The PainDETECT Questionnaire has been widely used to identify neuropathic components in chronic pain and has been validated for its reliability and validity across various cultural contexts.

From Lab to Life: Self-Powered Sweat Sensors and Their Future in Personal Health Monitoring.

The rapid development of wearable sweat sensors has demonstrated their potential for continuous, non-invasive disease diagnosis and health monitoring. Emerging energy harvesters capable of converting various environmental energy sources-biomechanical, thermal, biochemical, and solar-into electrical energy are revolutionizing power solutions for wearable devices. Based on self-powered technology, the integration of the energy harvesters with wearable sweat sensors can drive the device for biosensing, signal processing, and data transmission.

Exploring the role of CBLB in acute myocardial infarction: transcriptomic, microbiomic, and metabolomic analyses.

Background: Specific alterations in gut microbiota and metabolites have been linked to AMI, with CBLB potentially playing an essential role. However, the precise interactions remain understudied, creating a significant gap in our understanding. This study aims to address this by exploring these interactions in CBLB-intervened AMI mice using transcriptome sequencing, 16 S rDNA, and non-targeted metabolite analysis.

MIMU Optimal Redundant Structure and Signal Fusion Algorithm Based on a Non-Orthogonal MEMS Inertial Sensor Array.

A micro-inertial measurement unit (MIMU) is usually used to sense the angular rate and acceleration of the flight carrier. In this study, multiple MEMS gyroscopes were used to form a spatial non-orthogonal array to construct a redundant MIMU system, and an optimal Kalman filter (KF) algorithm was established by a steady-state KF gain to combine array signals to improve the MIMU's accuracy. The noise correlation was used to optimize the geometric layout of the non-orthogonal array and reveal the mechanisms of influence of correlation and geometric layout on MIMU's performance improvement.

Recent advances in metasurface design and quantum optics applications with machine learning, physics-informed neural networks, and topology optimization methods.

As a two-dimensional planar material with low depth profile, a metasurface can generate non-classical phase distributions for the transmitted and reflected electromagnetic waves at its interface. Thus, it offers more flexibility to control the wave front. A traditional metasurface design process mainly adopts the forward prediction algorithm, such as Finite Difference Time Domain, combined with manual parameter optimization.

Novel graphene electrochemical transistors incorporating zirconia inorganic molecular imprinted layer：Design, construction and application for highly sensitive and selective detection of acetaminophen.

Owing to their intrinsic amplifying effect together with chemical stability, graphene electrochemical transistor sensors (GECTs) are gaining momentum for sensing applications. However, the surface of GECTs for different detection substances must be modified with different recognition molecules, which was cumbersome and lack a universal method. Molecularly imprinted polymer (MIP) is a kind of polymer with specific recognition function for given molecules.

Potential values of formalin-fixed paraffin-embedded tissues for intratumoral microbiome analysis in breast cancer.

Breast cancer (BC) tissues have been proved to harbor microorganisms, which could potentially contribute to oncogenesis. Formalin-fixed paraffin-embedded (FFPE) tissues are the most widespread clinical samples in BC research. To verify the potential of FFPE tissues in microbiological analysis, we analyzed the microbial communities of FFPE and fresh frozen (FF) tumor samples from 30 participants diagnosed with BC deploying 16S rRNA sequencing.

Differential Expression of microRNAs and Target Genes Analysis in Olfactory Ensheathing Cell-derived Extracellular Vesicles Olfactory Ensheathing Cells.

Introduction: Olfactory ensheathing cells (OECs) are important transplantable cells for the treatment of spinal cord injury. However, information on the mechanism of OEC-derived extracellular vesicles (EVs) in nerve repair is scarce.

Methods: We cultured OECs and extracted the OEC-derived EVs, which were identified using a transmission electron microscope, nanoparticle flow cytometry, and western blotting.

miR-145a-5p/Plexin-A2 promotes the migration of OECs and transplantation of miR-145a-5p engineered OECs enhances the functional recovery in rats with SCI.

Background: Olfactory ensheathing cells (OECs) serve as a bridge by migrating at the site of spinal cord injury (SCI) to facilitate the repair of the neural structure and neural function. However, OEC migration at the injury site not only faces the complex and disordered internal environment but also is closely associated with the migration ability of OECs.

Methods: We extracted OECs from the olfactory bulb of SD rats aged <7 days old.

Correlation of R2* with fat fraction and bone mineral density and its role in quantitative assessment of osteoporosis.

Objectives: To investigate the correlation of R2* with vertebral fat fraction (FF) and bone mineral density (BMD), and to explore its role in the quantitative assessment of osteoporosis (OP).

Methods: A total of 83 patients with low back pain (59.77 ± 7.

Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression.

The aim of the present study was to elucidate the evolutionary trajectory of colon cells from normal colon mucosa, to adenoma, then to carcinoma in the same microenvironment. Normal colon, adenoma and carcinoma tissues from the same patient were analyzed by single-cell sequencing, which perfectly simulated the process of time-dependent colon cancer due to the same microenvironment. A total of 22 cell types were identified.

Graphene electrochemical transistor incorporated with gel electrolyte for wearable and non-invasive glucose monitoring.

With the rapid development of wearable electronic devices, health monitoring is undergoing a fundamental shift from hospital-centered treatment to patient-centered diagnosis. Solution-gated graphene transistors provide an effective platform for developing high-sensitivity wearable devices due to their unique signal amplification, low energy consumption, and compatibility for miniaturization. However, it is still a major challenge to perform real-time sweat composition monitoring directly on the dry skin surface.

Core-shell architectured NH-UiO-66@ZIF-8/multi-walled carbon nanotubes nanocomposite-based sensitive electrochemical sensor towards simultaneous determination of Pb and Cu.

Amino functionalized zirconium-based metal-organic framework (NH-UiO-66) and zinc-based zeolitic imidazolate framework (ZIF-8) were integrated to develop a core-shell architectured hybrid material (NH-UiO-66@ZIF-8, NU66@Z8). The morphology and structure evolutions of core-shell NU6@Z8 were investigated by FE-SEM, XRD, FTIR, and XPS. The NU66@Z8 combined with carboxylated multi-walled carbon nanotubes (CMWCNT) was deposited on a glassy carbon electrode (GCE) for fabricating an electrochemical platform towards detecting Pb and Cu.

Digital polymerase chain reaction duplexing method in a single fluorescence channel.

The digital polymerase chain reaction (dPCR) technique can quantify specific sequences of deoxyribonucleic acid using either a droplet-based or chip-based system. dPCR duplexing methods in a single fluorescence channel are typically based on the difference in fluorescence amplitude (F) between two targets. The different targets are distinguished from each other by the F-value variation using non-equal probe concentrations or different target lengths.

Accuracy and applicability of dual-energy computed tomography in quantifying vertebral bone marrow adipose tissue compared with magnetic resonance imaging.

Objectives: To evaluate the accuracy of dual-energy computed tomography (DECT) in quantifying bone marrow adipose tissue (BMAT) and its applicability in the study of osteoporosis (OP).

Methods: A total of 83 patients with low back pain (59.77 ± 7.

Vapor-Assisted In Situ Recrystallization for Efficient Tin-Based Perovskite Light-Emitting Diodes.

Tin-based perovskites are a promising candidates to replace their toxic lead-based counterparts in optoelectronic applications, such as light-emitting diodes (LEDs). However, the development of tin perovskite LEDs is slow due to the challenge of obtaining high-quality tin perovskite films. Here, a vapor-assisted spin-coating method is developed to achieve high-quality tin perovskites and high-efficiency LEDs.

The relationship between ambulatory arterial stiffness index and left ventricular diastolic dysfunction in HFpEF: a prospective observational study.

Background: The relationship between ambulatory arterial stiffness index (AASI) and left ventricular diastolic dysfunction (LVDD) in patients with heart failure with preserved ejection fraction (HFpEF) is unknown. We aimed to investigate the association between the AASI and LVDD in HFpEF.

Methods: We prospective enrolled consecutive patients with HFpEF in Chongqing, China.

Comparative analysis of the gut microbiota composition between knee osteoarthritis and Kashin-Beck disease in Northwest China.

Background: Osteoarthritis (OA) and Kashin-Beck disease (KBD) both are two severe osteochondral disorders. In this study, we aimed to compare the gut microbiota structure between OA and KBD patients.

Methods: Fecal samples collected from OA and KBD patients were used to characterize the gut microbiota using 16S rDNA gene sequencing.

Ag nanocubes monolayer-modified PDMS as flexible SERS substrates for pesticides sensing.

A new approach is presented to fabricate flexible surface-enhanced Raman scattering (SERS) substrate of Ag nanocubes monolayer-modified polydimethylsiloxane (Ag NCs/PDMS) through a powerful three-phase interface self-assemble method. The morphologies and crystal structures were characterized by scanning electron microscopy and X-ray diffraction. The self-assembled Ag NCs/PDMS substrate exhibited high SERS activity and good signal homogeneity, which was successfully used for quantitative detection of thiram; the detection limit reached 10 ng/mL, and the linear range is 10-1000 ng/mL.

Molecularly Controlled Quantum Well Width Distribution and Optoelectronic Properties in Quasi-2D Perovskite Light-Emitting Diodes.

Owing to their excellent optoelectronic properties, quasi-2D perovskites with self-assembled multiple quantum well (MQW) structures have shown great potential in light-emitting diode (LED) applications. Understanding the correlation between the bulky cation, quantum well assembly, and optoelectronic properties of a quasi-2D perovskite is important. Here, we demonstrate that the dipole moment of the bulky cation can be one of the fundamental factors that controls the distribution and crystallinity of different quantum wells.