Plasmonic colorimetry and G-quadruplex fluorescence-based aptasensor: A dual-mode, protein-free and label-free detection for OTA.

G-quadruplexes (G4) have received significant attention in the field of aptasensors owing to their unique physicochemical characteristics. A dual-mode, protein-free and label-free aptamer sensor based on plasmonic colorimetry and G4 fluorescence (PC@GF-aptasensor) was proposed for ochratoxin A (OTA). Colorimetry mode was achieved through the surface plasmon resonance (SPR) effect, which related to the OTA-Apt-based G4-OTA.

DNA-bridged double gold nanoparticles-based immunochromatography for dual-mode detection of ochratoxin A.

A novel immunochromatography strip (ICS) based on the DNA-bridged double gold nanoparticles was established and evaluated for the dual-mode detection of ochratoxin A (OTA). For this purpose, the streptavidin was coupled with the big size of gold nanoparticle (40 nm, AuNP), the OTA monoclonal antibody and biotinylated DNA-SH were simultaneously immobilized on the small size of AuNP (20 nm), and then the enhanced ICS was self-assembled and evaluated. The dual-mode detection of semi-quantification and quantification had been achieved and performed by the proposed ICS.