Tire wear particles enhance horizontal gene transfer of antibiotic resistance genes in aquatic ecosystems.

Microplastics (MPs) have introduced new surfaces for biofilm development and gene exchange among bacteria. We investigated Tire Wear Particles (TWPs) for their involvement in horizontal gene transfer (HGT), particularly in relation to associated metals in the matrices of TWPs. We employed red-fluorescently tagged E.

Matching N supply for yield maximization in salt-affected wheat agri-food systems: On-farm participatory assessment and validation.

Driven by the UN-SDGs of achieving food security and agricultural sustainability, it remains more challenging in degraded ecosystems to simultaneously improve the crop performance without creating unintended favour for excessive fertilization and associated environmental consequences. We assessed the N-use pattern of 105 wheat-growers in sodicity affected Ghaghar Basin of Haryana, India, and then experimented upon to optimize and identify indicators of efficient N use in contrasting wheat cultivars for sustainable production. The survey results revealed that majority of farmers (88%) have increased their reliance on N nutrition (∼18 % extra N), and even extended their duration of N scheduling (12-15 days) for better plant adaptation and yield insurance in sodicity stressed wheat; albeit to a greater extent in moderately sodic soils applying 192 kg N ha in 62 days.

Computational analysis and functional characterisation of Tor putitora toll-like receptor 4 with the elucidation of its binding sites for microbial mimicking ligands.

In the current study, full-length Toll-like receptor 4 (TLR4) cDNA was cloned and characterised in Tor putitora, an important fish inhibiting Himalayan rivers. The complete coding sequence of TpTLR4 is 2457 bp with nine key structural domains, including six leucine-rich repeats (LRRs). The phylogenetic tree revealed that TpTLR4 showed the closest relationship with TLR4 of Cyprinus carpio (96%), Labeo rohita (91%) and Megalobrama amblycephala (88%), all belonging to the Cyprinidae family.

Ecological and human health risk assessment of trace element pollution in sediments and five important commercial fishes of the Oman Sea.

The distribution of trace elements in sediments and five commercially important fish species of the Oman Sea were assessed using an atomic absorption spectrometer to determine ecological and human health risk assessment. The ranges of trace elements concentration in sediments were: Cr: 5.42-68.