Cinnamyl alcohol dehydrogenase downregulation in poplar wood increases saccharification after dilute acid pretreatment: a key role for lignin revealed by a multimodal investigation.

This study is the first to apply dilute acid pretreatment (DAP) under different severity conditions to poplar wood genetically modified for the cinnamyl alcohol dehydrogenase (CAD1) gene, which is involved in the lignin biosynthesis pathway. The carefully selected pretreatment conditions resulted in glucose yields that were 15 points higher for the hpCAD poplar line than for the wild-type (WT) wood after 48 h of enzymatic hydrolysis. To explain this higher saccharification rate, the chemical, spectral and structural changes in WT and hpCAD wood were analyzed in relation to the severity of the pretreatment process.