Synergistic Effect of Polyglycerol and DMSO for Long-Term Cryopreservation of Species.

Herein, we present a significant advancement in long-term cryopreservation techniques for microalgae species using a combination of linear polyglycerol (PG) and dimethyl sulfoxide (DMSO). The technique was tested on three species: , , and , which showed long-term viability and recovery rates superior to those when treated with a traditional cryoprotectant only. While DMSO alone enabled high cell recovery rates for all species after 1 week of cryopreservation, the rates for some of them dropped below 50% after 26 weeks of cryopreservation.

Analgesic Effect of SKI306X on Chronic Postischemic Pain and Spinal Nerve Ligation-Induced Neuropathic Pain in Mice.

Neuropathic pain (NP) results from lesions or diseases affecting the peripheral or central somatosensory system. However, there are currently no drugs that are particularly effective in treating this condition. SKI306X is a blend of purified extracts of three oriental herbs (Clematis mandshurica, Trichosanthes kirilowii, and Prunella vulgaris) commonly used to treat osteoarthritis for their chondroprotective effects.

The Halophilic Bacterium for the Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) from Single Carbon Sources.

The study objective was to evaluate the potential production of polyhydroxyalkanoates (PHAs), a biodegradable plastic material, by for which PHA production has never been reported. To identify the most effective nitrogen-limited culture conditions for PHAs production from this bacterium, batch fermentation using glucose concentrations ranging from 4 g l to 20 g l with a fixed ammonium concentration of 0.5 g l was carried out at 30°C and pH 8.

Valorization of carbon dioxide and waste (Derived from the site of Eutrophication) into syngas using a catalytic thermo-chemical platform.

Global warming increases a chance of eutrophication, and such fact offers that unhygienic organic waste materials (OWMs) in water must be treated. Hence, this study laid emphasis on the thermal-chemical (pyrolysis) process to establish a rapid valorization platform for OWMs. Indeed, OWMs were collected from the eutrophication site, and OWMs were mainly comprised of lignocellulosic biomass, microalgae (cyanobacteria) and the diverse types of bacteria (commonly observed from livestock waste).

Valorization of aflatoxin contaminated peanut into biodiesel through non-catalytic transesterification.

Aflatoxins (AFs) are the extremely hazardous metabolites (carcinogens) that are sporadically observed in crops, and these toxic chemicals are indeed lethal to the health of living organisms including human beings. Thus, AF contaminated food waste needs to be disposed as an environmentally benign way, not releasing it into the environment. This study offered a sustainable disposal and valorization platform for AF contaminated food.

Functional use of CO to mitigate the formation of bisphenol A in catalytic pyrolysis of polycarbonate.

The growing consumption of plastic materials has increased hazardous threats to all environmental media, since current plastic waste management methods release microplastics and toxic chemicals. As such, massive generation of plastic derived pollutants leads to significant public health and environmental problems. In this work, an environmentally sound method for valorization of plastic waste is suggested.

Pikeienuella piscinae gen. nov., sp. nov., a novel genus in the family Rhodobacteraceae.

A novel bacterium, designated strain RR4-56, was isolated from a biofilter of a seawater recirculating aquaculture system. The 16S rRNA gene sequence analysis showed that the isolate was closely related to Halovulum dunhuangense YYQ-30 (92.6%), Albimonas donghaensis DS2 (91.

Biodiesel synthesis from swine manure.

This study demonstrates that the biodiesel (BD) from swine manure (SM) could be a promising way for large scale generation of biofuel. Also, the economic and environmental benefits of SM derived BD were evaluated. Transesterification of lipid contents extracted from the collected SM had low BD yield (14.

gen. nov., sp. nov., a novel bacterium within the family .

A novel aerobic, Gram-negative bacterial strain, RR3-28, was isolated from a seawater recirculating aquaculture system in Busan, Republic of Korea. Cells were rod-shaped, non-motile, oxidase-positive, catalase-negative and grew optimally at 25-30 °C, pH 8.5 and 3 % (w/v) NaCl.

Production of bioplastic through food waste valorization.

The tremendous amount of food waste from diverse sources is an environmental burden if disposed of inappropriately. Thus, implementation of a biorefinery platform for food waste is an ideal option to pursue (e.g.

Effect of biochars pyrolyzed in N and CO, and feedstock on microbial community in metal(loid)s contaminated soils.

Little is known about the effects of applying amendments on soil for immobilizing metal(loid)s on the soil microbial community. Alterations in the microbial community were examined after incubation of treated contaminated soils. One soil was contaminated with Pb and As, a second soil with Cd and Zn.

[Fatal cardiac tamponade that developed in the post-anesthesia care unit: a rare complication after lung lobectomy].

Background And Objectives: Cardiac tamponade is potentially fatal medical condition, which rarely occurs as a complication of lung lobectomy. We present the first case of cardiac tamponade to develop in a Post-Anesthesia Care Unit following a lung lobectomy.

Case Report: A 54-year-old man with pulmonary squamous cell carcinoma underwent an apparently uncomplicated lung lobectomy.

Preventative effect of ketamine on post-surgical hyperalgesia induced at a body part remote from the surgical site.

Background: It is known that pain hypersensitivity can be induced at a body part remote from a surgical site (tertiary hyperalgesia), leading to patient discomfort. Nevertheless, no reported study to date has investigated methods to attenuate such tertiary hyperalgesia. Ketamine is known to modulate hyperalgesia induced by central sensitization.

The effect of humidified heated breathing circuit on core body temperature in perioperative hypothermia during thyroid surgery.

During general anesthesia, human body easily reaches a hypothermic state, which is mainly caused by heat redistribution. Most studies suggested that humidified heated breathing circuits (HHBC) have little influence on maintenance of the core temperature during early phase of anesthesia. This study was aimed at examining heat preservation effect with HHBC in case of undergoing surgery with less exposure of surgical fields and short surgical duration.

The role of algae and cyanobacteria in the production and release of odorants in water.

This review covers literatures pertaining to algal and cyanobacterial odor problems that have been published over the last five decades. Proper evaluation of algal and cyanobacterial odors may help establish removal strategies for hazardous metabolites while enhancing the recyclability of water. A bloom of microalgae is a sign of an anthropogenic disturbance in aquatic systems and can lead to diverse changes in ecosystems along with increased production of odorants.

Industrial robustness linked to the gluconolactonase from Zymomonas mobilis.

The physiological characteristics and the potential gluconolactone production of the gluconolactonase-deficient strain, Zymomonas mobilis ZM4 gnlΔ, were investigated via growth inhibitory assay and biotransformation of glucose and fructose into gluconolactone and sorbitol, respectively. The results of ethanol fermentation studies performed in the presence of high concentration of glucose (>200 g l) under fermentative or aerobic conditions indicated that a significant reduction of volumetric ethanol productivity from the strain of ZM4 gnlΔ was noticeable due to the reduced rates of specific growth, sugar uptake, and biomass yield as compared with those of the parental strain ZM4. The biotransformation prepared at pH 6.

Enhanced thermal destruction of toxic microalgal biomass by using CO2.

This work confirmed that dominant microalgal strain in the eutrophic site (the Han River in Korea) was Microcystis aeruginosa (M. aeruginosa) secreting toxins. Collected and dried microalgal biomass had an offensive odor due to microalgal lipid, of which the content reached up to 2±0.

Pyrogenic transformation of Nannochloropsis oceanica into fatty acid methyl esters without oil extraction for estimating total lipid content.

This study fundamentally investigated the pseudo-catalytic transesterification of dried Nannochloropsis oceanica into fatty acid methyl esters (FAMEs) without oil extraction, which was achieved in less than 5min via a thermo-chemical pathway. This study presented that the pseudo-catalytic transesterification reaction was achieved in the presence of silica and that its main driving force was identified as temperature: pores in silica provided the numerous reaction space like a micro-reactor, where the heterogeneous reaction was developed. The introduced FAME derivatization showed an extraordinarily high tolerance of impurities (i.

Pyrolysis of microalgal biomass in carbon dioxide environment.

This work mechanistically investigated the influence of CO2 in the thermo-chemical process of microalgal biomass (Chlorella vulgaris and Microcystis aeruginosa) to achieve a fast virtuous cycle of carbon via recovering energy. This work experimentally justified that the influence of CO2 in pyrolysis of microalgal biomass could be initiated at temperatures higher than 530 °C, which directly led to the enhanced generation of syngas. For example, the concentration of CO from pyrolysis of M.

Boosting the value of biodiesel byproduct by the non-catalytic transesterification of dimethyl carbonate via a continuous flow system under ambient pressure.

Transformation of coconut oil into biodiesel by using dimethyl carbonate (DMC) via a non-catalytic transesterification reaction under ambient pressure was investigated in this study. The non-catalytic transformation to biodiesel was achieved by means of a heterogeneous reaction between liquid triglycerides and gas phase DMC. The reaction was enhanced in the presence of porous material due to its intrinsic physical properties such as tortuosity and absorption/adsorption.

Effect of carbon dioxide on the thermal degradation of lignocellulosic biomass.

Using biomass as a renewable energy source via currently available thermochemical processes (i.e., pyrolysis and gasification) is environmentally advantageous owing to its intrinsic carbon neutrality.

Sequential co-production of biodiesel and bioethanol with spent coffee grounds.

The sequential co-production of bioethanol and biodiesel from spent coffee grounds was investigated. The direct conversion of bioethanol from spent coffee grounds was not found to be a desirable option because of the relatively slow enzymatic saccharification behavior in the presence of triglycerides and the free fatty acids (FFAs) found to exist in the raw materials. Similarly, the direct transformation of the spent coffee grounds into ethanol without first extracting lipids was not found to be a feasible alternative.

Synergetic sustainability enhancement via current biofuel infrastructure: waste-to-energy concept for biodiesel production.

The concept of waste-to-energy (WtE) with regards to the utilization of byproducts from the bioethanol industry (e.g., distiller's dried grain with solubles: DDGS) was employed to enhance the renewability of biodiesel, which would be an initiative stage of a biorefinery due to the conjunction between bioethanol and biodiesel.

Noncatalytic transformation of the crude lipid of ChlorellaI vulgaris into fatty acid methyl ester (FAME) with charcoal via a thermo-chemical process.

The noncatalytic transformation of the crude lipid of Chlorella vulgaris (C. vulgaris) into fatty acid methyl ester (FAME) via a thermo-chemical process was mainly investigated in this work. The crude lipid of C.

New candidate for biofuel feedstock beyond terrestrial biomass for thermo-chemical process (pyrolysis/gasification) enhanced by carbon dioxide (CO2).

The enhanced thermo-chemical process (i.e., pyrolysis/gasification) of various macroalgae using carbon dioxide (CO(2)) as a reaction medium was mainly investigated.