Development of microsatellite markers in Pungtungia herzi using next-generation sequencing and cross-species amplification in the genus Pseudopungtungia.

Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain reaction amplification. Of these primer sets, 16 new markers (16%) were successfully amplified with unambiguous polymorphic alleles in 16 individuals of Pungtungia herzi.

The complete mitochondrial genome of Kumgang fat minnow Rhynchocypris kumgangensis (Cypriniformes, Leuciscinae) in Korea.

The Kumgang fat minnow (Rhynchocypris kumgangensis) is an endemic and critically endangered freshwater species in the Korean peninsula. Its genome is 16604 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (CR). The gene order and composition of R.