Investigation of scaffold manufacturing conditions for 3-dimensional culture of myogenic cell line derived from black sea bream ().

Culturing fish myogenic cells in vitro holds significant potential to revolutionize aquaculture practices and support sustainable food production. However, advancement in in vitro culture technologies for skeletal muscle-derived myogenic cells have predominantly focused on mammals, with limited studies on fish. Scaffold-based three-dimensional (3D) culture systems for fish myogenic cells remain underexplored, highlighting a critical research gap compared to mammalian systems.

Wound healing effect of fucoidan-loaded gelatin/oxidized carboxymethyl cellulose hydrogel.

Various wound dressings are under development to accelerate wound healing, and hydrogels in particular have the potential to create ideal conditions for wound healing. In this study, we investigated a novel hydrogel based on gelatin/oxidized carboxymethyl cellulose loaded with fucoidan derived from Ecklonia cava (ECF) for wound treatment. The mechanical stability and self-healing ability of the hydrogel were optimized, and its cytocompatibility was demonstrated against RAW 264.

Marine polysaccharides for antibiofilm application: A focus on biomedical fields.

Microbial pathogens such as bacteria and fungi form biofilms, which represent substantial hurdles in treating human illness owing to their adaptive resistance mechanism to conventional antibiotics. Biofilm may cause persistent infection in a variety of bodily areas, including wounds, oral cavity, and vaginal canal. Using invasive devices such as implants and catheters contributes significantly to developing healthcare-associated infections because they offer an ideal surface for biofilm formation.

3D-printed polycaprolactone/collagen/alginate scaffold incorporating phlorotannin for bone tissue regeneration: Assessment of sub-chronic toxicity.

The development of effective scaffolds for bone regeneration is crucial given the increasing demand for innovative solutions to address bone defects and enhance healing process. In this study, a polycaprolactone/fish collagen/alginate (P/FC/A) 3D scaffold incorporating phlorotannin was developed to promote bone tissue regeneration. While the efficacy of the P/FC/A scaffold has been demonstrated through in vitro and in vivo experiments, its sub-chronic toxicity in animal models remains understudied, raising concerns regarding its safety in clinical application.

Control of Staphylococcus aureus infection by biosurfactant derived from Bacillus rugosus HH2: Strain isolation, structural characterization, and mechanistic insights.

Novel antimicrobials are urgently needed to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. This study explores the potential of biosurfactants derived from Bacillus rugosus HH2 as a novel antibacterial agent against MRSA. The biosurfactant, identified as surfactin, demonstrated surface-active properties, reducing surface tension to 37.

Detection of Mycotoxin Contamination in Foods Using Artificial Intelligence: A Review.

Mycotoxin contamination of foods is a major concern for food safety and public health worldwide. The contamination of agricultural commodities employed by humankind with mycotoxins (toxic secondary metabolites of fungi) is a major risk to the health of the human population. Common methods for mycotoxin detection include chromatographic separation, often combined with mass spectrometry (accurate but time-consuming to prepare the sample and requiring skilled technicians).

From Sea to Lab: Angiotensin I-Converting Enzyme Inhibition by Marine Peptides-Mechanisms and Applications.

To reveal potent ACE inhibitors, researchers screen various bioactive peptides from several sources, and more attention has been given to aquatic sources. This review summarizes the recent research achievements on marine peptides with ACE-inhibitory action and application. Marine peptides are considered excellent bioactives due to their large structural diversity and unusual bioactivities.

Artificial Intelligence-Driven Analysis of Antimicrobial-Resistant and Biofilm-Forming Pathogens on Biotic and Abiotic Surfaces.

The growing threat of antimicrobial-resistant (AMR) pathogens to human health worldwide emphasizes the need for more effective infection control strategies. Bacterial and fungal biofilms pose a major challenge in treating AMR pathogen infections. Biofilms are formed by pathogenic microbes encased in extracellular polymeric substances to confer protection from antimicrobials and the host immune system.

Effect of CdS loading on the properties and photocatalytic activity of MoS nanosheets.

Molybdenum sulfide (MoS) and modified MoS with different percentages of CdS (10%, 30%, and 50% CdS@MoS) were successfully synthesized and characterized. The photocatalytic performance of the MoS and CdS@MoS was evaluated by degrading brilliant green (BG), methylene blue (MB), and rhodamine B (RhB) dyes under visible light irradiation. Amongst the synthesized photocatalysts, 50% CdS@MoS exhibited the highest photocatalytic activity, degrading 97.

Microwave-assisted synthesis of ZnS@CuInS for photocatalytic degradation of coloured and non-coloured pollutants.

Copper indium sulfide (CuInS) exhibits strong visible light absorption and thus has the potential for good photocatalytic activity; however, rapid charge recombination limits its practical usage. An intriguing strategy to overcome this issue is to couple CuInS with another semiconductor to form a heterojunction, which can improve the charge carrier separation and, hence, enhance the photocatalytic activity. In this study, photocatalysts comprising CuInS with a secondary CuS phase (termed CuInS) and CuInS loaded with ZnS (termed ZnS@CuInS) were synthesized via a microwave-assisted method.

Microwave-assisted synthesis of Ni-doped europium hydroxide for photocatalytic degradation of 4-nitrophenol.

Microwave-assisted synthesis method was used to prepare europium hydroxide (Eu(OH)) and different percentages of 1, 5, and 10 % nickel-doped Eu(OH) (Ni-Eu(OH)) nanorods (NRs). X-ray diffraction study showed a hexagonal phase with an average crystallite size in the range of 21 - 35 nm for Eu(OH) and Ni-Eu(OH) NRs. FT-IR and Raman studies also confirmed the synthesis of Eu(OH) and Ni-Eu(OH).

Strategies for controlling polymicrobial biofilms: A focus on antibiofilm agents.

Polymicrobial biofilms are among the leading causes of antimicrobial treatment failure. In these biofilms, bacterial and fungal pathogens interact synergistically at the interspecies, intraspecies, and interkingdom levels. Consequently, combating polymicrobial biofilms is substantially more difficult compared to single-species biofilms due to their distinct properties and the resulting potential variation in antimicrobial drug efficiency.

Multifunctional Microneedle Patch with Diphlorethohydroxycarmalol for Potential Wound Dressing.

Background: Treatment of skin wounds with diverse pathological characteristics presents significant challenges due to the limited specific and efficacy of current wound healing approaches. Microneedle (MN) patches incorporating bioactive and stimulus materials have emerged as a promising strategy to overcome these limitations and integrating bioactive materials with anti-bacterial and anti-inflammatory properties for advanced wound dressing.

Methods: We isolated diphlorethohydroxycarmalol (DPHC) from Ishige okamurae and assessed its anti-inflammatory and anti-bacterial effects on macrophages and its antibacterial activity against Cutibacterium acnes.

Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms.

Pseudomonas aeruginosa is an opportunistic pathogen that produces two types of siderophores, pyoverdine and pyochelin, that play pivotal roles in iron scavenging from the environment and host cells. P. aeruginosa siderophores can serve as virulence factors and perform various functions.

Attenuation of biofilm and virulence factors of Pseudomonas aeruginosa by tetramethylpyrazine-gold nanoparticles.

Pseudomonas aeruginosa is often identified as the causative agent in nosocomial infections. Their adapted resistance makes them strong towards antimicrobial treatments. They protect and empower their survival behind strong biofilm architecture that works as their armor toward antimicrobial therapy.

Marine-derived bioactive materials as antibiofilm and antivirulence agents.

Microbial infections are major human health issues, and, recently, the mortality rate owing to bacterial and fungal infections has been increasing. In addition to intrinsic and extrinsic antimicrobial resistance mechanisms, biofilm formation is a key adaptive resistance mechanism. Several bioactive compounds from marine organisms have been identified for use in biofilm therapy owing to their structural complexity, biocompatibility, and economic viability.

Impact of Co-Doping on the Visible Light-Driven Photocatalytic and Photoelectrochemical Activities of Eu(OH).

The microwave-assisted synthesis approach was used to synthesize Eu(OH) and Co-Eu(OH) nanorods. Various techniques were used to investigate the structural, optical, and morphological features of the Eu(OH) and Co-Eu(OH) NRs. Both Eu(OH) and Co-Eu(OH) NRs were found to be hexagonal with crystallite sizes ranging from 21 to 35 nm.

Photocatalytic degradation of brilliant green and 4-nitrophenol using Ni-doped Gd(OH) nanorods.

Gadolinium hydroxide (Gd(OH)) was synthesized via a microwave-assisted synthesis method. Nickel ion (Ni) was doped into Gd(OH), in which 4-12% Ni-Gd(OH) was synthesized, to study the effect of doping. The structural, optical, and morphological properties of the synthesized materials were analyzed.

Bactericidal effect of water-washing methods on contaminated in a raw fish : water type, temperature, and pH.

This study aimed to evaluate a method for effectively reducing contamination in fish based on the type of washing water and method. Texture profiles and sensory evaluations were performed to determine the effect of the developed method on the quality and preference of the samples. The selected fish sample was , which is mainly consumed in Asian countries.

Silver nanoparticles synthesized from pyoverdine: Antibiofilm and antivirulence agents.

The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane.

Bacterial extracellular vesicles: Modulation of biofilm and virulence properties.

Microbial pathogens cause persistent infections by forming biofilms and producing numerous virulence factors. Bacterial extracellular vesicles (BEVs) are nanostructures produced by various bacterial species vital for molecular transport. BEVs include various components, including lipids (glycolipids, LPS, and phospholipids), nucleic acids (genomic DNA, plasmids, and short RNA), proteins (membrane proteins, enzymes, and toxins), and quorum-sensing signaling molecules.

Optimization of Protease Treatment Conditions for Protein Extraction and Investigation of Its Potential as an Alternative Protein Source.

This study aimed to determine enzymes that effectively extract proteins and optimize the processing conditions using response surface methods. Furthermore, the potential of enzymatically hydrolyzed protein extract (CPE) as a substitute protein source was investigated. The enzymatic hydrolysis conditions for protein extraction were optimized using single-factor analysis and a response surface methodology-Box-Behnken design.

A literature review of bioactive substances for the treatment of periodontitis: , and clinical studies.

Periodontitis is a common chronic inflammatory disease of the supporting tissues of the tooth that involves a complex interaction of microorganisms and various cell lines around the infected site. To prevent and treat this disease, several options are available, such as scaling, root planning, antibiotic treatment, and dental surgeries, depending on the stage of the disease. However, these treatments can have various side effects, including additional inflammatory responses, chronic wounds, and the need for secondary surgery.