Lower trapezius myocutaneous flap repairs adjacent deep electrical burn wounds.

Background: Local tissue damage caused by electrical burns is often deep and severe. High-voltage electrical burns are common in the head, neck and torso areas. These are mostly caused by direct contact with the power supply and are often accompanied by deep injuries of the nerve, blood vessel, muscle, tendon, and bone.

HIF-1α Enhances Vascular Endothelial Cell Permeability Through Degradation and Translocation of Vascular Endothelial Cadherin and Claudin-5 in Rats With Burn Injury.

The mechanism underlying burn injury-induced enhanced vascular endothelial permeability and consequent body fluid extravasation is unclear. Here, the rat aortic endothelial cells (RAECs) were treated with the serum derived from rats with burn injury to elucidate the mechanism. Sprague-Dawley (SD) rats were grouped as follows (10 rats/group): control, 2, 4, 8, 12, and 24 hours postburn groups.

Effects of hypoxia inducible factor-1α on expression levels of MLCK, p-MLC and ZO-1 of rat endothelial cells.

Objective: To examine the aberrant expression of endothelial permeability associated proteins including MLCK, p-MLC and ZO-1 in presence of different levels of hypoxia-inducible factor 1 alpha (HIF-1α).

Methods: We established monolayer vascular endothelial cell model with the primary rat endothelial cells. Over-expressed or under-expressed HIF-1α cell lines were made by endothelial cells transfected with plasmid vector constructed with HIF-1α gene or HIF-1α-specific short hairpin RNA (shRNA).

Self-Gating: An Adaptive Center-of-Mass Approach for Respiratory Gating in PET.

The goal is to develop an adaptive center-of-mass (COM)-based approach for device-less respiratory gating of list-mode positron emission tomography (PET) data. Our method contains two steps. The first is to automatically extract an optimized respiratory motion signal from the list-mode data during acquisition.