Antimicrobial Resistance in Isolated from Food Workers and Chicken Products in Japan.

is an enteric bacterial pathogen that causes foodborne illness in humans. Third-generation cephalosporin (TGC) resistance in remains a global concern. Food workers may represent a reservoir of , thus potentially contaminating food products.

[Detection of enteric pathogen bacteria with the PCR method from mixed fecal specimens of food handlers].

We developed an assay for rapid, specific detection of Shigella, Salmonella, and verotoxin-producing Escherichia coli O157 using the direct PCR analysis of mixed human fecal specimens. In this study, the sensitivity of the direct PCR assay for 50 mixed human fecal specimen was found to be 1.3, 0.

[Direct PCR detection of food-borne bacteria from mixed human feces].

Mixed human feces were evaluated for simultaneous direct PCR detection of 3 food-borne bacteria--verotoxin-producing bacteria, Salmonella, and Shigella. Mixed feces concentrated approximately 2.5% in distilled water, were heated at 95 degrees C for 5 min.