3D structured illumination microscope using a spinning disk [Invited].

Three-dimensional (3D) structured illumination microscopy (SIM) improves spatial resolution by a factor of two in both lateral and axial directions. However, the adoption of 3D SIM is limited by low imaging speed, susceptibility to out-of-focus light, and likelihood of reconstruction errors. Here we present a novel approach for 3D SIM using a spinning disk.

Structured illumination microscopy with a phase-modulated spinning disk for optical sectioning.

Among various super-resolution microscopic techniques, structured illumination microscopy (SIM) stands out for live-cell imaging because of its higher imaging speed. However, conventional SIM lacks optical sectioning capability. Here we demonstrate a new, to the best of our knowledge, approach using a phase-modulated spinning disk (PMSD) that enhances the optical sectioning capability of SIM.

The nanoscale organization of Nipah virus matrix protein revealed by super-resolution microscopy.

The matrix proteins (M) of many enveloped RNA viruses mediate virus assembly and budding. However, it remains poorly understood how M are involved in virus budding and how they interact with envelope proteins. Here, we show that the expression level of Nipah (NiV) M in particles produced by the host cells deviates from a gamma distribution and does not reflect that of the host cells, indicating assembly of the NiV-M in the process.

The DFT Quest for Possible Reaction Pathways, Catalytic Species, and Regioselectivity in the InCl-Catalyzed Cycloaddition of -Tosyl Formaldimine with Olefins or Allenes.

The present work focuses on a theoretical investigation of the plausible mechanism, determination of catalytically active species, and understanding of the regioselectivity in the InCl-catalyzed cycloaddition of -tosyl formaldimine with alkenes or allenes. InCl and InCl coordinated by dichloroethane (InCl-DCE) were investigated as model catalytic systems. DFT data supported that InCl-DCE represent the plausible in situ generated catalytic species.

Identification of associated SSR markers for yield component and fiber quality traits based on frame map and Upland cotton collections.

Detecting QTLs (quantitative trait loci) that enhance cotton yield and fiber quality traits and accelerate breeding has been the focus of many cotton breeders. In the present study, 359 SSR (simple sequence repeat) markers were used for the association mapping of 241 Upland cotton collections. A total of 333 markers, representing 733 polymorphic loci, were detected.

[Epidemiologic research on the clinical features of patients with cervical spondylosis].

Objective: To investigate the clinical features of patients with cervical spondylosis.

Methods: Questionnaires were provided and X rays were examined in 1 009 people with different occupations, ages, and sexes.

Results: All the patients were diagnozed as cervical spondylosis.