Molecular detection and genetic characteristics of porcine reproductive and respiratory syndrome virus in central China.

Porcine reproductive and respiratory syndrome caused by porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating viral diseases in the global pork industry. To further clarify the epidemic characteristics of the virus, 365 clinical samples were collected from diseased pigs suffering from abortion and respiratory disease from 2018 to 2023 on 63 pig farms in Henan and Shanxi provinces, and screened for the presence of PRRSV using reverse transcription-polymerase chain reaction (RT-PCR). A total of 62 clinical samples (62/365, 16.

Integrated ribosome and proteome analyses reveal insights into sevoflurane-induced long-term social behavior and cognitive dysfunctions through ADNP inhibition in neonatal mice.

A growing number of studies have demonstrated that repeated exposure to sevoflurane during development results in persistent social abnormalities and cognitive impairment. Davunetide, an active fragment of the activity-dependent neuroprotective protein (ADNP), has been implicated in social and cognitive protection. However, the potential of davunetide to attenuate social deficits following sevoflurane exposure and the underlying developmental mechanisms remain poorly understood.

Molecular detection and genetic characteristics of a novel porcine circovirus (porcine circovirus 4) and porcine reproductive and respiratory syndrome virus in Shaanxi and Henan Provinces of China.

Porcine circovirus 4 (PCV4) is a recently discovered circovirus that was first reported in 2019 in several pigs with severe clinical disease in Hunan province of China, and also identified in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV). To further investigate the epidemic profile and genetic characteristics of the two viruses, 150 clinical samples were collected from 9 swine farms in Shaanxi and Henan provinces of China, and a SYBR Green I-based duplex quantitative real-time polymerase chain reaction (qPCR) was developed for detecting PCV4 and PRRSV simultaneously. The results showed the limits of detection were 41.

Simultaneous detection and phylogenetic analysis of porcine epidemic diarrhea virus and porcine circovirus 4 in Henan province, China.

Porcine circovirus 4 (PCV4) is a recently discovered circovirus that was first reported in 2019 in several pigs in Hunan province of China and has also been identified in pigs infected with porcine epidemic diarrhea virus (PEDV). To further investigate the coinfection and genetic diversity of these two viruses, 65 clinical samples (including feces and intestinal tissues) were collected from diseased piglets on 19 large-scale pig farms in Henan province of China, and a duplex SYBR Green I-based quantitative real-time polymerase chain reaction (qPCR) assay was developed for detecting PEDV and PCV4 simultaneously. The results showed that the limit of detection was 55.

[Chemical Constituents of Ethyl Acetate Fraction of Suaeda glauca].

Objective: To study the chemical constituents of Suaeda glauca.

Methods: The chemical constituents were isolated and purified with several separation and purification techniques. Their structures were identified by physicochemical properties and various spectroscopic methods.

[Study on Chemical Constituents from Roots of Lonicera macranthoides].

Objective: To study chemical constituents of the roots of Lonicera macranthoides.

Methods: The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods.

Results: Seven compounds were isolated and identified as ribenol (1), excoecarin C (2), 18-hydroxy-13-epi-manoyloxide (3), asiatic acid (4), oleanolic acid (5), β-sitosterol (6) and β-daucosterol (7).

[Water-soluble chemical constituents in flower buds of Lonicera macranthoides].

Objective: To study the water-soluble chemical constituents of the flower buds of Lonicera macranthoides.

Methods: The chemical constituents were isolated and purified by means of chromatographic techniques and their structures were elucidated by spectroscopic methods.

Results: Six compounds were isolated and identified as trans-linalool-3,7-oxide-6-O-beta-D-glucopyranoside(1), secol oganoside(2), secoxyloganin(3),chlorogenic acid(4), caffeic acid(5), sucrose(6).

[Study on chemical constituents of the bulbs of Lycoris longituba].

Objective: To study the chemical constituents of the Bulbs of Lycoris longituba.

Methods: The chemical constituents were isolated and purified by means of chromatographic techniques and their structures were elucidated by spectroscopic methods.

Results: Six compounds were isolated and identified as Crinine (1), Narciclasine (2), Squamigine (3), 11-Hydroxyvittatine (4), Pancracine (5), Lycorine (6).

[Studies on the chemical constituents of Salicornia europaea].

Objective: To study the chemical constituents of Salicornia europaea.

Methods: The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods.

Results: Five compounds were isolated and identified as syringaresinol 4-O-beta-D-glucopyranoside(1), icariside B2(2), erythro-1-(4-O-beta-D-glucopyranosyl-3,5-dimethyoxyphenyl)-2-syringaresinoxyl-propane-1,3-diol(3), longifloroside B(4), 7-O-beta-D-glucopyranosyl-6-methoxychromone(5).

[Studies on the chemical constituents of the aerial part of Bupleurum chinense (II)].

Objective: To study the chemical constituents of the aerial part of Bupleurum chinense.

Methods: Dried aerial parts of Bupleurum chinense were extracted with EtOH, the chemical constituents in the extract were isolated and their structures were identified by physical and chemical properties and spectral analysis.

Results: Five compounds were obtained and identified as 1-O-caffeoylglycerol (1), esculetin (2), scopoletin (3), kaempferol-3-O-beta-D-rutinoside (4) and alpha-spinastero (5).