[Establishment and optimization of sliver staining differential display of microtubers from Pinellia ternata in vitro].

Objective: In this study, orthogonal design was used to optinize DDRT-PCR amplification system on Pinellia ternata microtubers in vitro in five factors four levels respectively.

Method: P. ternata stems and microtubers in vitro were selected as explants.

[Efficient method for extraction of high quality RNA from microtubers of Pinellia ternata in vitro].

Objective: To extract RNA from Pinellia ternata and lay a foundation for studying the formation mechanism of P. ternata.

Method: By modifying the method recommended by Guanidinium for extracting total RNA from plant tissues rich in phenolic and polysaccharidic compounds, a simple and convenient method for extraction of total RNA from the tubers, stems and leaves of P.

[Levels of amino acids in cerebral spinal fluid in children with cerebral palsy].

Objective: To study the changes of amino acids in cerebral spinal fluid (CSF) in children with spastic or athetotic cerebral palsy (CP) by examining CSF levels of glutamic acid (Glu), gamma-aminobutyric acid (GABA) and aspartate (ASP).

Methods: CSF samples were obtained from 13 children with spastic CP, from 14 children with athetotic CP, and from 10 children without central nervous system and infectious diseases (control group). CSF levels of Glu, GABA and ASP were determined by high-performance liquid chromatography.