Publications by authors named "You-fu Xie"
Increased CD4CD45RAFoxP3 cells alter the balance between Treg and Th17 cells in colitis mice.
World J Gastroenterol
November 2016
Article Synopsis
- The study aimed to explore the balance between regulatory T cells (Tregs) and T helper 17 (Th17) cells in mice suffering from colitis, particularly how different Treg subsets play a role in this balance.
- Using flow cytometry and real-time PCR, researchers assessed various immune cell populations and cytokine levels in mouse tissues affected by ulcerative colitis.
- Results showed an increase in certain Treg subsets and Th17 cells in the colon tissue, suggesting that an imbalance between these cell types could be significant in colitis pathology, particularly in the lamina propria of the colon.
[Expression of secretions of hypothalamus-pituitary-adrenal axis in human hypertrophic scar].
Zhonghua Shao Shang Za Zhi
December 2011
Objective: To explore the expression and significance of secretions of hypothalamus-pituitary-adrenal (HPA) axis in human hypertrophic scar.
Methods: Hypertrophic scar tissues obtained from 12 patients with deep-partial thickness burn or full-thickness burn and normal skin tissues from the same 7 patients with hypertrophic scar were harvested for determination of gene expression of corticotrophin-releasing hormone (CRH), CRH receptor 1 (CRH-R1), pro-opiomelanocortin (POMC), melanocortin receptor 2 (MC-2R), and glucocorticoid receptor α (GR-α) by real-time fluorescence quantitative PCR. After addition of corresponding antibodies, distribution differences of CRH, CRH-R1, adrenocorticotropic hormone (ATCH), MC-2R, and GR-α were observed with immunohistochemical staining.
[Effect of melatonin on proliferation and apoptosis of fibroblasts in human hypertrophic scar].
Zhonghua Shao Shang Za Zhi
December 2011
Objective: To study the effect of melatonin on proliferation and apoptosis of fibroblasts in human hypertrophic scar and its mechanism.
Methods: Fibroblasts from human hypertrophic scar were isolated and cultured with DMEM medium containing 10% FBS, and then they were divided into control (C, added with ethanol), low concentration (LC, added with 1 × 10(-5) mmol/L melatonin), middle concentration (MC, added with 1 × 10(-3) mmol/L melatonin), and high concentration (HC, added with 1 mmol/L melatonin) groups according to the random number table. After being cultured for 24 hours, cell morphologic change was observed under microscope; XTT-PMS assay was used to examine cell proliferative activity; cell cycle and apoptosis were assessed with flow cytometry after double staining of FITC and PI, and the levels of cyclin E, p53, and Fas mRNA were determined with fluorescence quantitative RT-PCR.
[The expression of melatonin receptor in human hypertrophic scar].
Zhonghua Zheng Xing Wai Ke Za Zhi
May 2010
Objective: To investigate the expression and its significance of melatonin receptor in human hypertrophic scarring.
Methods: The expression of melatonin receptor GPR50 was detected with immunohistochemistry and the melatonin receptors (MT1, MT2) mRNA were assessed with RT-PCR method in 10 cases of human hypertrophic scar and normal skin. The positive production was sequenced with auto sequencing instrument.
Management of early complications of the electrical injury and repair of the injured hands.
Di Yi Jun Yi Da Xue Xue Bao
October 2002
Objective: To explore the management of early complications and repair of hand injuries in electrically injured patients.
Methods: Forty-five patients with electrical injuries admitted during 1995 and 2001 were analyzed retrospectively in terms of the incidences of early complications and the deformity of the injured hands repaired using different approaches.
Results: In the order of incidence, the complications arising in the early stages after electrical injuries included electric shock, hypovolemic shock, cardiac dysfunction, hemorrhage of the digestive tract, pulmonary infection, massive bleeding of the wound and renal dysfunction.