Publications by authors named "You-fei Qi"
We aimed to explore the effects of single nucleotide polymorphisms (SNPs) in tropoelastin gene on tropoelastin mRNA and elastin expressions in human aortic smooth muscle cells (HASMCs). Two SNP loci, rs2071307 (G/A) and rs1785598 (G/C), were selected to construct recombinant lentivirus vectors carrying wild-type and mutant tropoelastin gene. Recombinant plasmids including pWSLV-02-ELN, pWSLV-02-ELN-mut1, and pWSLV-02-ELN-mut2 were constructed, before being amplified by polymerase chain reaction (PCR) and sequenced.
View Article and Find Full Text PDFBackground: Vascular calcification (VC), as a prevalent feature of atherosclerosis (AS), is a life-threatening pathological change. Mitofusin 2 (MFN2) has been reported to be down-regulated and participate in the pathogenesis of AS. Here, we explored the feasible impacts of MFN2 on VC in AS.
View Article and Find Full Text PDFCircHIPK3 Regulates Vascular Smooth Muscle Cell Calcification Via the miR-106a-5p/MFN2 Axis.
J Cardiovasc Transl Res
December 2022
Atherosclerosis is the most common arterial disease and is closely related to vascular calcification. CircHIPK3 has been implicated in atherosclerosis development, but the possible downstream regulatory mechanisms remain unclear. The levels of circHIPK3, miR-106a and MFN2 in tissues and blood samples of patients with atherosclerosis were detected by RT-qPCR.
View Article and Find Full Text PDFArticle Synopsis
- - Aortic dissection (AD) is a severe condition that results in high death and illness rates, linked primarily to problems with the elastin-coding gene called tropoelastin (TE), but other factors may also contribute to its development.
- - This study explored how microRNAs (specifically miR-144-3p) influence the levels of elastin by targeting TE mRNA in aortic smooth muscle cells, revealing that miR-144-3p can inhibit TE protein production.
- - Findings indicated that AD patients had higher levels of miR-144-3p and lower levels of TE in their aortic smooth muscle cells, while treatment in a mouse model that counteracted miR-144-
Background: Infected abdominal aortic aneurysms (iAAAs) are rare but life-threatening diseases. The purpose of the present study was to report our experience of extra-anatomic prosthesis bypass in the retroperitoneum as a treatment for iAAAs.
Methods: Data of 8 consecutive patients diagnosed with iAAAs and treated by an extra-anatomic prosthesis bypass in the retroperitoneum were retrospectively collected.
Endoscopic variceal ligation combined with Hassab's procedure in preventing the recurrence of esophageal varices.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
June 2007
Objective: To assess the efficacy of endoscopic variceal ligation (EVL) combined with Hassab's procedure in the prevention of variceal recurrence.
Methods: One hundred and thirty-five patients with esophageal varices were randomized to receive EVL alone, Hassab's procedure alone or a combination of EVL and Hassab's procedure for variceal eradication. Ultrasonographic venous network images were recorded by an esophageal microprobe before and after the EVL or Hassab's procedure.