Direct proteomic mapping of Streptomyces avermitilis wild and industrial strain and insights into avermectin production.

An industrial mutant of Streptomyces avermitilis produced avermectin at a high level in industrial complex culture medium. However, almost no avermectin was detected in the cultures of tryptone soya broth (TSB). Its wild-type strain could not synthesize avermectin.

Does urothelial cancer of bladder behave differently in young patients?

Background: Bladder urothelial cancer has been diagnosed at an increasing rate among young adults in China while the clinical outcomes remain highly controversial. To optimize the management of young patients with bladder cancer, we examined whether bladder urothelial cancer in young patients behaved differently from that in the elder patients.

Methods: From 1994 to 2008, a database of bladder urothelial cancer patients at a major tertiary medical center was retrospectively reviewed.

[Effect of polygona-polysaccharids on atheromatous plaque: experiment with rabbits].

Objective: To study the effect of polygona-polysaccharids on the elimination of atheromatous plaque.

Methods: Thirty adult New Zealand rabbits were fed with high-fat and cholesterol forage for 8 weeks to establish hyperlipidemia animal models and then randomly divided into 3 equal groups: model group, simvastatin group receiving simvastatin orally, and polygona-polysaccharids group receiving polygona-polysaccharids orally. Ten rabbits were fed with normal food as control forage.

[Effects of temperature on the kinetics and level of energy charge and oxidation-reduction state in pyruvate biosynthesis].

Temperature plays an important role in pyruvate biosynthesis by Torulopsis glabrata 620. The effects of temperature on the substrate consumption, cell growth, pyruvate biosynthesis and level of energy charge and oxidation-reduction state have been investigated. During the constant temperature fermentation, higher temperature can enhance the rate of glucose consumption, cell growth and pyruvate production.

[LC-ESI/MS identification and reduction of impurities in spiramycin fermentation].

There are several impurities in the spiramycin fermentation broth which leads to a lower yield and lower quality of the product. Four impurities in spiramycin broth have been simultaneously separated and identified by LC-ESI/MS. The generation of these impurities was attributed to the fluctuation of glucosylation in spiramycin biosynthesis.

[Determination of organic acids in fermentation broth of spiramycin by high performance liquid chromatography].

A method for determining organic acids in spiramycin fermentation broth by high performance liquid chromatography is described. The operating conditions were Zorbax 300-SB C18 column (5 microns, 4.6 mm i.