Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34).

Objectives: To investigate the technical performance of IDentifier DNA typing kit (YanHuang34) and evaluate its forensic application value.

Methods: Following the (GB/T 37226-2018), IDentifier DNA typing kit (YanHuang34) was verified in 11 aspects of species specificity, veracity, sensibility, adaptability, inhibitor tolerance, consistency, balance, reaction condition verification, mixed samples, stability and inter batch consistency. The system efficiency of IDentifier DNA typing kit (YanHuang34) was compared with the PowerPlex Fusion 6C System, VersaPlex 27PY System and VeriFiler Plus PCR Amplification Kit.

[DNA extraction from bones and teeth using AutoMate Express forensic DNA extraction system].

Objective: To explore a new method in order to extract DNA from bones and teeth automatically.

Methods: Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system.

[Application and progress of RNA in forensic science].

With the development of molecular biology, the evidences of genetics has been used widely in forensic sciences. DNA technology has played an important role in individual identification and paternity testing, RNA technology is showing more and more wide application in prospect. This article reviews the application and progress of RNA in forensic science including estimation of postmortem interval, bloodstain age, wound age, as well as determination of cause of death and the source of body fluids.