Effects of magnetic field exposure patterns on polyhydroxyalkanoates synthesis by Haloferax mediterranei at extreme hypersaline context: Carbon distribution and salt tolerance.

The synthesis of polyhydroxyalkanoates (PHAs) by extremophiles presents a promising alternative to mitigate pollution originating from the use of petroleum-based plastics. This study focuses on the impact of different magnetic field (MF) exposure patterns on PHA production and carbon metabolism, aiming to enhance PHA productivity by Haloferax mediterranei within the extreme hypersaline environment and subsequently reducing production costs. Results indicated that under 300 g/L salinity, the highest PHA productivity (1.

Simultaneous aerobic nitrogen and phosphorus removal by novel halotolerant fungus Mucor circinelloides SNDM1: Function and metabolism pathway.

Fungi capable of simultaneous nitrogen and phosphorus removal from wastewater is rarely found. Here, a novel fungal strain (SNDM1) performing heterotrophic nitrification, aerobic denitrification, and phosphate removal was isolated and identified as Mucor circinelloides. The favorable nutrient removal conditions by the strain using glucose were C/N ratios of 25-30, salinities of 0 %-3 %, and pH of 7.

Mutualistic symbiosis of fungi and nitrogen-fixing bacteria in halophilic aerobic granular sludge treating nitrogen-deficient hypersaline organic wastewater.

Hypersaline organic wastewater is characterized as being nitrogen-deficient, and is easily prone to sludge bulking. In this study, the stability of halophilic aerobic granular sludge (HAGS) for the treatment of hypersaline organic wastewater is explored. Along with the decrease of influent ammonium, the bacterial population substantially reduced, whereas the fungal population continuously increased in HAGS.

Simultaneous inorganic nitrogen and phosphate removal by aerobic-heterotrophic fungus Fusarium keratoplasticum FSP1: Performance, pathway and application.

Fungi with multiple contaminant removal function have rarely been studied. Here, a novel fungal strain Fusarium keratoplasticum FSP1, which was isolated from halophilic granular sludge, is reported for first time to perform simultaneous nitrogen and phosphate removal. The strain showed wide adaptability under C/N ratios of 30-35, salinities of 0 %-3 % (m/v), and pH of 7.

Feast/famine ratio regulates the succession of heterotrophic nitrification-aerobic denitrification and autotrophic ammonia oxidizing bacteria in halophilic aerobic granular sludge treating saline wastewater.

Heterotrophic nitrification-aerobic denitrification (HN-AD) shows innovation potential of wastewater treatment process in a single tank. However, how to enrich HN-AD bacteria in activated sludge to enhance their contribution remained unknown. This study explored the impact of the feast/famine (F/F) ratio on the succession of autotrophic ammonia oxidizing bacteria (AOB) and HN-AD bacteria in a halophilic aerobic granular sludge (HAGS) system.

Simultaneous treatment of epichlorohydrin wastewater and polyhydroxyalkanoate recovery by halophilic aerobic granular sludge highly enriched by Halomonas sp.

The treatment of epichlorohydrin (ECH) wastewater exists chances for achieving cleaner production. This study initially employed moderately halophilic aerobic granular sludge (HAGS) to treat ECH wastewater, and the resulting HAGS was utilized to recover polyhydroxyalkanoate (PHA). During the acclimation process of HAGS, the chemical oxygen demand removal efficiency stabilized at 70 %.

Static magnetic field increases aerobic nitrogen removal from hypersaline wastewater in activated sludge with coexistence of fungi and bacteria.

Fungi have been found to exist in activated sludge treating saline wastewater, but their role in removing pollution has been neglected. This study explored the aerobic removal of total inorganic nitrogen (TIN) from saline wastewater under static magnetic fields (SMFs) with several strengths. Compared to the control, the aerobic removal of TIN was significantly increased by 1.

A halophilic aerobic-heterotrophic strain Halomonas venusta SND-01: Nitrogen removal by ammonium assimilation and heterotrophic nitrification-aerobic denitrification.

Nitrogen (N) removal from high-salinity wastewater is a major challenge. The aerobic-heterotrophic nitrogen removal (AHNR) process has been demonstrated to be feasible for treating hypersaline wastewater. In this study, Halomonas venusta SND-01, a halophilic strain capable of performing AHNR, was isolated from saltern sediment.

Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation.

There is very limited research on the application of moderate halophiles for biotreatment of hypersaline wastewater widely generated from some industries. This study demonstrated the development of moderate halophiles inoculated from saltern sediments into aerobic granule sludge (AGS) to treat hypersaline wastewater with a salinity of 100 g/L. The granulation of moderate halophiles can occur without applying the settling velocity selective pressure.

A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal.

Nitrogen and phosphate removal from wastewater relies on different functional bacteria. In this study, a novel strain affiliated with Pseudomonas aeruginosa was isolated from activated sludge by gradient dilution and performed heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal (HNADPR). The strain showed an ammonium removal efficiency of 87% and a phosphate removal efficiency of 97% under optimal conditions, such as C/N ratio of 10, P/N ratio of 0.

Coupling Magnetic Field and Salinity Upshock To Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater.

Low polyhydroxyalkanoate (PHA) volumetric productivity from wastewater limits low-cost PHA production. To resolve this problem, an external magnetic field (MF) coupled with upshock salinity was applied to PHA production by Haloferax mediterranei (family ). Elevating the fermentation salinity over the optimal growth salinity (200 g/L) increased the PHA cell content while inhibiting cell proliferation, decreasing volumetric productivity.

Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects.

Sulfur-driven autotrophic denitrification (SADN) is a promising technology for nitrogen removal from wastewater. In this study, different-strength SMFs (0, 5, 20, 50, 70 mT) were evaluated to investigate the potential of external static magnetic field (SMF) for enriching sulfur-oxidizing autotrophic denitrifiers (SOAD). 50-mT and 70-mT SMFs were most suitable to accelerate the growth of SOAD and the elimination of non-SOAD.

Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon.

Suitability of different substrates for enriched mixed microbial cultures (MMCs) is of importance to the polyhydroxyalkanoate (PHA) fermentation using renewable carbon. In this study, three enriched MMCs were evaluated for their fermentation features and kinetics with different carbon sources (sodium acetate, glucose, or starch). The results showed that the highly specific bacterial community composition was developed depending on the applied carbon source.

A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification.

The utilization of halophilic bioresources is limited due to a lack of isolation and characterization work. A halophilic bacterium strain SND-01 of Exiguobacterium mexicanum was isolated in this study, which is the first report on its novel function in heterotrophic nitrification-aerobic denitrification (HN-AD). The strain SND-01 is slightly halophilic, surviving at 0 up to 9% (w/v) salinity.

The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity.

How polymer synthesis is mobilized or activated as a biological response of Haloferax mediterranei against hypertonic conditions remains largely unexplored. This study investigated the protein expression of H. mediterranei in response to high salinity by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis.

Fast granulation of halophilic activated sludge treating low-strength organic saline wastewater via addition of divalent cations.

Granulation of halophilic activated sludge is an important solution to solve the problem of solid-liquid separation in biological treatment of saline wastewater. This study demonstrated that by adding divalent cations into the saline influent with low organic load, halophilic granular sludge with an average diameter of 910 ± 10 μm can be cultivated. The close correlation between divalent cations and particle size indicated that Ca played a major role in the granulation process.

Granulation of halophilic sludge inoculated with estuarine sediments for saline wastewater treatment.

As a solution of the sludge loss in the treatment of saline wastewater, the granulation of halophilic sludge was explored in this study. The inoculated estuarine sediment was granulated to an average diameter of 1155 ± 102 μm under the selective settling pressure in the airlift sequencing batch reactor (SBR) when the influent organic loading rate (OLR) was doubled to 0.36 g COD/L·day.

[Effect of Carbon Sources on the Accumulation of Endogenous Polymers and Denitritation in the O/A-F/F Mode].

To accumulate endogenous polymers during the aerobic phase, the aerobic/anoxic-feast/famine (O/A-F/F) selection mode can be used. It can also be used for endogenous denitrification by activated sludge during the anoxic phase. To further explore the effect of carbon sources on the activated sludge accumulation of endogenous polymers and endogenous denitrification, this study used acetic and glucose as the main carbon sources to investigate the accumulation of endogenous polymers, endogenous denitrification, and the structure and function of enriched activated sludge.

Cr(VI) Adsorption on Red Mud Modified by Lanthanum: Performance, Kinetics and Mechanisms.

Water pollution caused by the highly toxic metal hexavalent chromium (Cr(VI)) creates significant human health and ecological risks. In this study, a novel adsorbent was used to treat Cr(VI)-containing wastewater; the adsorbent was prepared using red mud (RM) generated from the alumina production industry and the rare earth element lanthanum. This study explored adsorption performance, kinetics, and mechanisms.

Effects of carbon sources on the enrichment of halophilic polyhydroxyalkanoate-storing mixed microbial culture in an aerobic dynamic feeding process.

Microbial polyhydroxyalkanoate (PHA) production serves as a substitute for petroleum-based plastics. Enriching mixed microbial cultures (MMCs) with the capacity to store PHA is a key precursor for low-cost PHA production. This study investigated the impact of carbon types on enrichment outcomes.

The effects of salinity on nitrification using halophilic nitrifiers in a Sequencing Batch Reactor treating hypersaline wastewater.

With annual increases in the generation and use of saline wastewater, the need to avoid environmental problems such as eutrophication is critical. A previous study identified ways to start up a halophilic sludge domesticated from estuarine sediments to remove nitrogen from wastewater with a salinity of 30 g/L. This investigation expands that work to explore the impact of salinity on nitrogen removal.

[Salt effects on treatment performance and microbial community structure in MUCT].

It is essential for a full-scale treatment plant processing saline wastewater to understand the effects of salt on an activated sludge system and the performance of pollutant removal. For this purpose, a bench-scale modified university of cape town (MUCT) process was tested to treat real saline sewage with salt concentrations of 5, 8, 10 and 15 g/L. The experimental results indicated that salt in sewage significantly affected nutrient removal compared with organic removal.

Achieving and maintaining biological nitrogen removal via nitrite under normal conditions.

The principal aim of this paper is to develop an approach to realize stable biological nitrogen removal via nitrite under normal conditions. Validation of the new method was established on laboratory-scale experiments applying the sequencing batch reactor (SBR) activated sludge process to domestic wastewater with low C/N ratio. The addition of sodium chloride (NaCl) to influent was established to achieve nitrite build-up.