Publications by authors named "You-Lan Shan"
Adefovir dipivoxil (ADV) is used as first-line monotherapy or rescue therapy in chronic hepatitis B (CHB) patients. In this study, we sought to identify nucleotide changes in the reverse transcriptase (RT) of hepatitis B virus (HBV) at baseline and explore their predictive value for ADV antiviral response. Ultra-deep pyrosequencing (UDPS) was utilized to determine HBV genetic variability within the RT region at baseline and during a 48-week ADV therapy.
View Article and Find Full Text PDFWe compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.
View Article and Find Full Text PDFHepatitis B virus (HBV) is an important cause of human chronic liver diseases and is a major public health problem. Viral load and HBV genotype play critical roles in determining clinical outcomes and response to antiviral treatment in hepatitis B patients. Viral genotype detection and quantification assays are currently in use with different levels of effectiveness.
View Article and Find Full Text PDFArticle Synopsis
- This study aimed to investigate the causes of pneumococcal meningitis and identify potential drug targets.
- Researchers used a promoter-trap library and infected mice to create a meningitis model, analyzing bacteria that activated under these conditions.
- They identified 52 genes linked to essential functions like adherence and metabolism, highlighting new targets for understanding and treating pneumococcal meningitis.
Assessment of a method for detecting serum HBV DNA with HBV DNA probe labelled directly by alkaline phosphatase.
Hepatobiliary Pancreat Dis Int
November 2003
Objective: To assess a sensitive and specific technique for detecting serum HBV DNA with an HBV DNA probe labelled directly by alkaline phosphatase (AlkPhos Direc probe).
Methods: AlkPhos Direc probe was prepared with purified HBV DNA labelled directly by alkaline phosphatase. The probe and chemiluminescent substrate CDP-star for AP were used in hybridization assay.