Publications by authors named "You-Fen Dai"
Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1.
Bioelectrochemistry
February 2018
The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode.
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- Extracellular polymeric substances (EPS) are important for the formation and survival of bacterial bio-aggregates, specifically in Shewanella oneidensis MR-1.
- Five extraction methods were tested, with heating at 40°C and EDTA treatment being the most effective, producing good EPS yields while minimizing damage to bacterial cells.
- The analyses showed that heating at 40°C yielded 7.12 mg of proteins and 1.60 mg of polysaccharides per gram of dry cell, while EDTA provided slightly lower yields but resulted in minimal cell lysis.