Androgen down-regulated and region-specific expression of germ cell nuclear factor in mouse epididymis.

Germ cell nuclear factor (GCNF), a nuclear orphan receptor, involved in spermatogenesis, neurogenesis, differentiation, and embryo development, was highly expressed with two transcripts (7.4 and 2.3 kb) in mouse testis and with only one transcript (7.

Identification of the Subunits of C3P4 and Estimation of Their Molecular Weights by UV Cross-linking Assay.

The subunits and their molecular weights of an unknown DNA-binding protein C3P4 induced by androgen were studied by UV cross-linking assay. The results indicate that there are two subunits with molecular weights of about 110 kD and 27 kD in the C3P4 complex. Ferguson plot further shows the molecular weight of native C3P4 to be about 127 kD.

Identification of differentially expressed genes in human uterine leiomyomas using differential display.

Unlabelled: In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis.

Studies of the Expression of Progesterone Receptor Gene in the Rat Uterus during Early Pregnancy by RT-PCR.

Progesterone plays an important role in the establishment and maintenance of pregnancy. However, there have been no systematic reports on the expression of the rat uterine progesterone receptor (PR) gene during this process. In the present studies PR mRNA levels in rat uterus were analyzed during the estrous cycle and early pregnancy (d1-d10), using reverse transcription-polymerase chain reaction (RT-PCR).

The Interaction of Recombinant Androgen Receptor with the Androgen Responsive Element.

A 1 119 bp (1105 to 2224) fragment of androgen receptor (AR) cDNA, named AR1 (containing the whole DNA binding domain, the hinge site region and the partial hormone binding domain) was constructed into an expression vector pGEX. The GST-ARI fusion protein was expressed in E. coli induced by IPTG and purified from glutathione-Sepharose-4B affinity column.

The Tissue Distribution of a New Androgen Inducible Cytosol Protein.

As a preliminary but important step in the investigation of the function of the new androgen-dependent DNA binding cytosol protein (C(3)P(4)) discovered by using gel retardation assay with a 31 bp fragment of DNA of the promoter of the rat ventral prostatic binding protein (PSBP) C(3) gene as a probe, a general survey was carried out on the tissue distribution of this protein in rat mice, rabbit and monkey of both sexes and also in some human pathological tissues with the following findings: (1) C(3)P(4) is present in all the male sex organs in different amount, but none in female sex organs. (2) C(3)P(4) has been found in the brain of all the species studied. (3) Its occurrence in other tissues are rare and show species variation, but no sex difference.

An Unknown Protein Binding Specifically to the Coding Strand of the Estrogen Responsive Element.

An unknown protein, Designated ERE-C-SSBP, which binds to the single coding strand of estrogen response element (ERE) tightly and specifically, has been discovered unexpectedly during an attempt to establish an ER-ERE gel retardation assay with a 13 bp core fragment of the ERE as a probe. Some of its characteristics, such as subcellular location, tissue distribution, heat stability and relation with divalent metal ions are reported. Interestingly, it may be down-regulated by estrogen.