[Analysis of animal models of stable chronic obstructive pulmonary disease based on clinical features of traditional Chinese medicine and western medicine].

This study analyzed the advantages and disadvantages of existing animal models in China and abroad and their goodness of fit based on the clinical characteristics and diagnostic criteria of stable chronic obstructive pulmonary disease(COPD) in traditional Chinese medicine(TCM) and western medicine, followed by the collation and summarization of model evaluation methodologies. The results showed that the existing animal models of stable COPD were mainly modeled via smoke exposure or the combination of multiple methods like smoke exposure plus lipopolysaccharide or protease or bacterial infection. These animal models generally failed to simulate the clinical characteristics of TCM, and their goodness of fit in western medicine was higher than that in TCM.

Wentong decoction cures allergic bronchial asthma by regulating the apoptosis imbalance of EOS.

Background: Eosinophils (EOS) is one of the most important cells involved in the pathogenesis of chronic airway inflammation in asthma, and its apoptosis is part of the mechanisms of asthma. Therefore, this study aimed to observe the effect of Chinese medicine Wentong decoction (WTD) in EOS apoptosis in asthmatic rats. This work also explored the mechanism of WTD regulation in EOS apoptosis and provided a new target for clinical treatment of asthma.

Structural Modulation of Gut Microbiota in Rats with Allergic Bronchial Asthma Treated with Recuperating Lung Decoction.

Objective: To investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment.

Methods: Fifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group.

Flax seed oil inhibits metastatic melanoma and reduces lung tumor formation in mice.

Purpose: Cancer is one of the leading causes of mortality worldwide. Tumor cells circulating in the blood evidence the migration of tumor from the site of origin to another site leading to the formation of new metastatic lesion and establishment of metastatic tumors. In the present study, cultured metastatic tumor cells were injected into the C57BL/6 mice through tail-vein injection (TVI) and the anti-metastatic properties of flax seed oil (FSO) were evaluated.

Studies of the microbial metabolism of flavonoids extracted from the leaves of Diospyros kaki by intestinal bacteria.

Flavonoid glycosides are metabolized by intestinal bacteria, giving rise to a wide range of phenolic acids that may exert systemic effects in the body. The microbial metabolism of flavonoids extracted from the leaves of Diospyros kaki (FLDK) by intestinal bacteria was investigated in vitro. High-performance liquid chromatography/linear trap quadrupole orbitrap mass spectrometry was performed to analyze the metabolites of flavonoids in vivo using Xcalibur2.

[Development of a patient-reported outcome scale for stable chronic obstructive pulmonary disease and its clinical applicability].

Objective: To develop a patient-reported outcome (PRO) scale for patients with stable chronic obstructive pulmonary disease (sCOPD) and to study its clinical applicability in traditional Chinese medicine (TCM).

Methods: According to standardized procedures, the test version of PRO scale for sCOPD (sCOPD-PRO scale) was developed based on the theory of "unity of body and spirit" and was used to test 324 patients selected from six clinical centers. Five methods were used to analyze the items; the reliability, validity and responsiveness of the remaining items of the scale were evaluated.

[Diagnostic significance of multi-slice computed tomography imaging in congenital inner ear malformations].

Objective: To evaluate the feasibility and usability of multi-slice computed tomography (MSCT) in congenital inner ear malformations.

Methods: Fourty-four patients with sensorineural hearing loss (SNHL) were examined by a Somatom Sensation 16 (siemens, Germany) CT scanner with following parameters: 120 kV, 100 mAs, 0.75 mm collimation, 1 mm reconstruction increment, a pitch factor of 1 and a field of view of 100 mm.

Immunologic role of nitric oxide in acute rejection of golden hamster to rat liver xenotransplantation.

Aim: To evaluate the immunologic role and expression significances of nitric oxide(NO), nitric oxide synthase(NOS),and its isoenzyme in acute rejection to liver xenografts from golden hamster in rat.

Methods: Liver transplantations were randomly divided into five groups(n=6-9):isografts (group I); xenografts (group II); xenografts plus cyclosporine treatment (group III), xenografts plus cyclophosphamide treatment combined with splenectomy (group IV), and xenografts using cyclophosphamide in combination with splenectomy (group IV) and xenografts using splenectomy in addition to cyclophosphamide and cyclosporine treatments(group V). The levels of ALT,TNF-alpha, and nitric oxide production(NOx) in serum of reciprents were examined,and expressions of type II(iNOS) and type III (cNOS) nitric oxide synthase(NOS)-inducible NOS(iNOS) and constitutive NOS(cNOS) were observed by NADPH diaphorase histochemical and immunohistochemical staining.