[The optimal resuscitation pressure of several resuscitation fluids in controlling hemorrhagic shock in rats].

Objective: To investigate the optimal mean blood pressure of different resuscitation fluids in resuscitating hemorrhagic shock in rats.

Methods: One hundred and eighty Sprague-Dawley (SD) rats were used to reproduce hemorrhagic shock model by 35% and 45% depletion of blood volume, and they were randomly divided into Lactated Ringer solution (LR), 7.5% NaCl/6% Dextran 40 (HSD), and LR+hydroxyethyl starch (HES) groups.

[Changes in systemic and local vascular reactivity in hemorrhagic shock and the therapeutic effect of delta opioid receptor antagonist ICI174,864].

Objective: To investigate the changes in systemic and local vascular reactivity following hemorrhagic shock of different severity and the therapeutic effect of alpha opioid receptor antagonist ICI174,864.

Methods: Fifty-six Wistar rats were used in two experiments. In experiment I, 32 rats were equally divided into sham operation group, 1 hour, 2 hours and 3 hours hypotension groups.

[The inflammatory mediators induced endothelial cell injury through delay neutrophils apoptosis].

This study aimed at elucidating the relationship between NTP apoptosis and endothelial cell injury. After isolation and purification, activated NTPs were incubated with endothelial cells directly or indirectly contact in a ratio of 5:1 and 10:1, meanwhile 10ng/ml IL-6 and 10% (v/v) burned serum added or not. Apoptosis and necrosis of endothelial cells were detected in situ.

[Mechanism of better result of limited resuscitation in a model of uncontrolled hemorrhagic shock].

Objective: To investigate the mechanism of better result of limited resuscitation in a model of uncontrolled hemorrhagic shock.

Methods: Uncontrolled hemorrhagic shock was produced in 54 rats by a standardized massive splenic injury with transection of the middle branch of splenic artery (MSIA). The rats were randomly assigned to six groups (n=6) by maintenance of the level of mean arterial pressure (MAP): sham-operated group (SS), 40 (RS40), 50 (RS50), 60 (RS60), 80 (RS80) and 100 mm Hg (RS100, 1 mm Hg=0.

PMN apoptosis and its relationship with the lung injury after chest impact trauma.

Background: Polymorphonuclear neutrophil (PMN), one of the most important inflammatory cells, functions throughout the initiation, progression and resolution of inflammation. This study aimed at investigating the relationship between PMN apoptosis and the lung injury after chest impact trauma.

Methods: PMNs were purified from rabbits subjected to the chest impact trauma and their apoptosis, necrosis, survival and respiratory burst were detected by flow cytometry.

Effect of initial fluid resuscitation on subsequent treatment in uncontrolled hemorrhagic shock in rats.

Using a modified uncontrolled hemorrhage shock model with massive splenic and vascular injury, we evaluated outcome and tissue oxidation injury with different resuscitation interventions during prehospital and hospital phases. The aim of our study was to explore the effect of initial fluid resuscitation on subsequent treatment of uncontrolled hemorrhagic shock in rats. Uncontrolled hemorrhagic shock was produced in 114 Wistar rat by sharp transection both of the splenic parenchyma at one location between the major branches of the splenic artery into the spleen and of one of the major branches of the splenic artery.