Genome-wide regulatory gene-derived SSRs reveal genetic differentiation and population structure in fiber flax genotypes.

We designed a set of 580 simple sequence repeat markers; 506 from transcription factor-coding genes, and 74 from long non-coding RNAs and designated them as regulatory gene-derived simple sequence repeat (ReG-SSR) markers. From this set, we could anchor 559 ReG-SSR markers on 15 flax chromosomes with an average marker distance of 0.56 Mb.

Perovskite-type BiFeO/ultrathin graphite-like carbon nitride nanosheets p-n heterojunction: Boosted visible-light-driven photoelectrochemical activity for fabricating ampicillin aptasensor.

Developing effective sensing method for trace analysis of ampicillin (AMP) is urgent and significant due to its residue possess serious threats to human health. Herein, a p-n heterojunction, on the basis of p-type BiFeO nanoparticles coupled n-typed ultrathin graphite-like carbon nitride (utg-CN) nanosheets, has been designed and synthesized via a simple electrostatic interaction strategy. Such p-n heterojunction has two advantages: one is capable to narrow the band gap of photoactive materials from 2.

Mechanisms of Zuogui Pill in Treating Osteoporosis: Perspective from Bone Marrow Mesenchymal Stem Cells.

The current treatment strategies for osteoporosis (OP) involve promoting osteogenic differentiation and inhibiting adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). According to a theory of traditional Chinese medicine (TCM), the kidneys contain an "essence" that regulate bone metabolism and generate marrow. Kidney disorders are therefore considered to be a major cause of OP as per the principles of TCM, which recommends kidney-tonifying treatments for OP.

Erratum: First-in-man clinical trial of CAR NK-92 cells: safety test of CD33-CAR NK-92 cells in patients with relapsed and refractory acute myeloid leukemia.

[This corrects the article on p. 1083 in vol. 8, PMID: 30034945.

Muscle fibre type composition in the lateral muscle of olive flounder Paralichthys olivaceus.

In this paper, a combined-method study has been made on the lateral muscle of the teleost olive flounder Paralichthys olivaceus in just-hatched and adult stages. In just-hatched stage, both slow and fast muscle fibres were detected: (1) in situ hybridization analysis indicated that slow and fast myosin heavy chain genes were specifically expressed in the superficial and deep part of the myotomal muscle, respectively; (2) immunohistochemistry analysis showed that fibres in the deep part reacted with anti-fast myosin antibody F310; (3) western blot analysis detected a weak expression of slow myosin and a strong expression of fast myosin. In adult stage, the slow and fast muscle fibres had their own distribution characteristics: (1) hematoxylin/eosin staining showed the histological characteristics of the muscle fibre composition; (2) histochemical observations showed that the deep muscle fibres, and some fibres near the epidermis, contain alkali-stable myofibrillar ATPase activity; (3) immunohistochemistry analysis indicated that all the deep muscle fibres reacted with F310 antibody and some fibres in the superficial layer of muscle also reacted with F310; (4) western blot analysis showed that fast myosin was expressed both in the blended muscles (the mix of superficial and deep muscles) and deep muscles, while slow myosin was mainly expressed in the blended muscles.

Characterization and expression of androgen receptors in olive flounder.

Androgens are critical hormones that regulate sex differentiation, sexual maturation, and spermatogenesis in vertebrates, which is mainly mediated by androgen receptors (ARs). Reports on transcript variants of ar (AR encoding gene) in human are almost always associated with cancers and androgen insensitivity syndrome. However, the knowledge of ar variants in teleosts is scarce.

Study of ciprofloxacin removal by biochar obtained from used tea leaves.

In this study, used tea leaves (UTLs) were pyrolyzed to obtain used tea-leaf biochar (UTC), and then the UTC was used as an adsorbent to remove ciprofloxacin (CIP) from aqueous solutions. Batch experiments were conducted to investigate the CIP adsorption performance and mechanism. The results showed that the CIP-adsorbing ability first increased and then declined as the UTC pyrolysis temperature increased.

Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3¦Â and C-myc.

Objective: To investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).

Methods: HPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX).

Genome-Wide Association Analysis of Mucilage and Hull Content in Flax ( L.) Seeds.

New flaxseed cultivars differing in seed mucilage content (MC) with low hull content (HC) represent an attractive option to simultaneously target the food and feed markets. Here, a genome-wide association study (GWAS) was conducted for MC and HC in 200 diverse flaxseed accessions genotyped with 1.7 million single nucleotide polymorphism (SNP) markers.

A facile strategy for modifying boron nitride and enhancing its effect on the thermal conductivity of polypropylene/polystyrene blends.

Boron nitride (BN) possesses excellent thermal conductivity and remarkable insulating properties. However, poor compatibility between BN fillers and a polymer matrix and the weak ultimate mechanical properties of polymer composites are still big challenges to industrial applications in the thermal conductive field. In this paper, the dispersion of BN in a polystyrene (PS) matrix can be improved through the surface modification of BN by introducing dispersion of polystyrene.

Weipixiao attenuate early angiogenesis in rats with gastric precancerous lesions.

Background: Angiogenesis is a pathobiological hallmark of gastric cancer. However, rare studies focus on angiogenesis in gastric precancerous lesions (GPL). Weipixiao (WPX), a Chinese herbal preparation, is proved clinically effective in treating GPL.

Characteristics of during Gonad Differentiation of the Olive Flounder .

The P450 side-chain cleavage enzyme, P450scc (Cyp11a) catalyzes the first enzymatic step for the synthesis of all steroid hormones in fish. To study its roles in gonads of the olive flounder , an important maricultured fish species, we isolated the genomic DNA sequence of 1396 bp, which consists of 5 exons and 4 introns. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) results indicated that the flounder was exclusively expressed in gonad and head kidney tissues.

A novel combination of quick response code and microfluidic paper-based analytical devices for rapid and quantitative detection.

Nowadays, there is great interest in the use of microfluidic paper-based analytical devices (μPADs) for low-cost diagnostics. In most cases, the test equipment is needed. Here we report a new type of device-independent detection method based on timer-paper-based analytical devices, which can be tested by smartphone, and its application for cholesterol detection.

Detection and phylogenetic analysis of porcine bocaviruses carried by murine rodents and house shrews in China.

Bocaparvovirus infections of humans and both wild and domestic animals have been widely reported around the world. In this study, we detected and genetically characterized porcine bocavirus (PBoV) carried by murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus losea) and house shrews (Suncus murinus) in China. Between May 2015 and May 2017, 496 murine rodents and 23 house shrews were captured in four Chinese provinces.

Synergistic Enhancement of Thermal Conductivity and Dielectric Properties in Al₂O₃/BaTiO₃/PP Composites.

Multifunctional polymer composites with both high dielectric constants and high thermal conductivity are urgently needed by high-temperature electronic devices and modern microelectromechanical systems. However, high heat-conduction capability or dielectric properties of polymer composites all depend on high-content loading of different functional thermal-conductive or high-dielectric ceramic fillers (every filler volume fraction ≥ 50%, i.e.

Comparative performance of growth, vertebral structure and muscle composition in diploid and triploid Paralichthys olivaceus.

Growth, skeletal structure and muscle composition of cold-shock-induced triploid olive flounder Paralichthys olivaceus were investigated. The average values of total length and total weight of triploids were higher than those of diploids from 5 to 11 months posthatch (mph). The growth difference disappeared after 11 mph.

Spermatogonial stem cells differentiation and testicular lobules formation in a seasonal breeding teleost: The evidence from the heat-induced masculinization of genetically female Japanese flounder (Paralichthys olivaceus).

Germ cells undergo a series of cellular changes including differentiation, mitosis, meiosis and maturation, and eventually develop into a large number of functional gametes. The available data regarding teleost gametogenesis in seasonal batch spawners is limited. In this study, we investigated spermatogenesis with special attention on spermatogonia differentiation using heat-induced masculine juveniles of genetically female Japanese flounder.

MiR-221 negatively regulates innate anti-viral response.

The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner.

Genome-Wide Association Study and Selection Signatures Detect Genomic Regions Associated with Seed Yield and Oil Quality in Flax.

A genome-wide association study (GWAS) was performed on a set of 260 lines which belong to three different bi-parental flax mapping populations. These lines were sequenced to an averaged genome coverage of 19× using the Illumina Hi-Seq platform. Phenotypic data for 11 seed yield and oil quality traits were collected in eight year/location environments.

Presence of rat bocavirus in oropharyngeal and fecal samples from murine rodents in China.

In this study, we detected and genetically characterized rat bocavirus (RBoV) carried in 496 murine rodents that were captured in four provinces in China between May 2015 and May 2017. RBoV-positive samples were found in all four provinces, with a total positive rate of 24.8% (123/496) in throat swabs and 58.

First-in-man clinical trial of CAR NK-92 cells: safety test of CD33-CAR NK-92 cells in patients with relapsed and refractory acute myeloid leukemia.

CAR T cells have shown clinical efficacy for acute lymphoblastic leukemia, but this therapy has not been effective for acute myeloid leukemia (AML), and other treatment options are needed. Theoretically, CAR-NK cells have a more favorable toxicity profile compared to CAR T cells, especially in avoiding adverse effects such as cytokine release syndrome. However, the clinical evidence for this has not yet been reported.

DNA barcoding of marine fish species from Rongcheng Bay, China.

Rongcheng Bay is a coastal bay of the Northern Yellow Sea, China. To investigate and monitor the fish resources in Rongcheng Bay, 187 specimens from 41 different species belonging to 28 families in nine orders were DNA-barcoded using the mitochondrial cytochrome c oxidase subunit I gene (). Most of the fish species could be discriminated using this COI sequence with the exception of and The average GC% content of the 41 fish species was 47.

[Preparation of Adsorption Ceramsite Derived from Sludge Biochar].

Adsorption ceramsite (SKC) was prepared with biochar (BC) derived from municipal sludge and kaolin (KL) based on the optimized processing parameters to adsorb ciprofloxacin (CIP) in aqueous solutions. The CIP adsorption mechanism of SKC was investigated by adsorption kinetics and isotherm adsorption models, combined with the analysis of microstructure, pore structure, phase composition, and zeta potential. Furthermore, the heavy metal leaching toxicity was assessed using the toxicity characteristic leaching procedure (TCLP) method.

CSF miR-16 expression and its association with miR-16 and serotonin transporter in the raphe of a rat model of depression.

Background: Depression is a common mental disorder with unknown mechanism. Emerging evidence shows that miRNAs play a critical role in the process of depression. Here we reported the cerebrospinal fluid (CSF) miR-16 expression and its association with miR-16 and serotonin transporter (SERT) in the raphe of a rat model of depression.