Multibubble sonoluminescence pulses from Na atoms in viscous liquid.

Multibubble sonoluminescence pulses of Na and continuum emissions were measured from NaCl-ethylene glycol solution saturated with Xe at 28 kHz. The Na emission consisted of multiple-peak pulses and single pulses. The intrinsic pulse width estimated from single pulses was 0.

Activation of transient receptor potential ankyrin 1 by hydrogen peroxide.

Hydrogen peroxide (H(2)O(2)), which is contained in industrial products, is also generated within cells. H(2)O(2) causes pain but it has not been elucidated how it activates sensory neurons in the pain pathway. Here we show that transient receptor potential ankyrin 1 (TRPA1), expressed by sensory neurons in the pain pathway, is a receptor for H(2)O(2).

Cold sensitivity of recombinant TRPA1 channels.

TRPM8 and TRPA1, members of the transient receptor potential (TRP) channel family, are candidates for cooling-activated receptors. It is accepted that TRPM8 responds to moderate cooling, although it is controversial whether TRPA1 responds to deep cooling. Here, using Ca(2+) imaging and/or patch-clamp recordings, we examined the thermal sensitivity of primary cultured dorsal root ganglion (DRG) neurons and mouse TRPA1-expressing human embryonic kidney (HEK) 293 cells.

Ca2+-dependent PKC activation mediates menthol-induced desensitization of transient receptor potential M8.

In 1950, Hensel and Zotterman reported cooling-induced desensitization of cold receptors by extracellular discharge recordings of cold fibers. Since then, however, its intracellular mechanism has remained unresolved. We studied menthol-induced desensitization of cold/menthol receptors (TRPM8, transient receptor potential M8) expressed in HEK cells.

TRPM8 protein localization in trigeminal ganglion and taste papillae.

TRPM8 is a TRP family cation channel which can be activated by cold stimuli or l-menthol. However, TRPM8 protein localization of nerve terminals in sensory organs remains unknown. Here we generated an antibody against TRPM8 and analyzed TRPM8 protein localization in trigeminal ganglia (TG) and in sensory nerve fibers in the tongue.