Publications by authors named "Yoshitake Sakai"
Article Synopsis
- In vitro studies of germ-cell development using pluripotent stem cells (PSCs) in mice and humans reveal important differences in their developmental mechanisms, indicating the need for similar studies in non-human primates.
- Researchers successfully maintained embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from cynomolgus monkeys in an undifferentiated state and efficiently induced them into primordial germ cell-like cells (PGCLCs).
- The induction of PGCLCs from cynomolgus monkey PSCs occurs faster than in humans, with significant differences in transcriptome dynamics compared to mouse PGCLCs, paving the way for comparative studies of germ-cell development across species.
Germline specification underlies human reproduction and evolution, but it has proven difficult to study in humans since it occurs shortly after blastocyst implantation. This process can be modeled with human induced pluripotent stem cells (hiPSCs) by differentiating them into primordial germ cell-like cells (hPGCLCs) through an incipient mesoderm-like cell (iMeLC) state. Here, we elucidate the key transcription factors and their interactions with important signaling pathways in driving hPGCLC differentiation from iPSCs.
View Article and Find Full Text PDF