Publications by authors named "Yoshitaka Nakauchi"
Article Synopsis
- Opalescence in therapeutic antibody solutions poses a challenge in drug formulation due to unclear underlying mechanisms.
- The study explored how antibody concentration affects the assembly states of bovine gamma globulin and human immunoglobulin G, revealing submicron-scale networks that caused a transparent blue opalescence without forming visible precipitates.
- The inclusion of stabilizers like trehalose and arginine was found to reduce this opalescence, offering valuable insights for enhancing protein formulations.
Opalescence is a problem concerned with the stability of an antibody solution. It occurs when a high concentration of a protein is present. Arginine (Arg) is a versatile aggregation suppressor of proteins, which is among the candidates that suppress opalescence in antibody solutions.
View Article and Find Full Text PDFLong-term storage of proteins at ambient temperature is required for applications in pharmaceutics and biotechnology. Lyophilization is a versatile approach for stabilizing proteins at ambient temperature, although its freezing and drying processes negatively affect the protein structure. In this study, we show a glass-like protein condensate (GLPC) as a new method for protein stabilization at ambient temperature.
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