Clinical significance of cerebrospinal fluid inhibitory titers of antibiotics, based on pharmacokinetic/pharmacodynamic parameters, in the treatment of bacterial meningitis.

The cerebrospinal fluid (CSF) inhibitory titer (CSF-IT) of an antibiotic, which can be used to estimate the duration of time above the agent's MIC in the CSF, was introduced as one of the indices to evaluate the effectiveness of antibiotic selection in treating bacterial meningitis. The CSF-IT was determined via a microdilution method. A suspension of the causative organism was added to a tube containing twofold diluted CSF and double-concentrated Mueller-Hinton broth with supplement.

Genetic features of clinical isolates of Streptococcus dysgalactiae subsp. equisimilis possessing Lancefield's group A antigen.

Thirteen Streptococcus dysgalactiae subsp. equisimilis isolates possessing Lancefield's group A antigen recovered from people in Japan during 2000 to 2004 were genotyped. The results indicate that a conserved clone has persisted and spread within Japan, and two different emm types were observed within members of this clone.

In vitro activities of piperacillin against beta-lactamase-negative ampicillin-resistant Haemophilus influenzae.

The in vitro activities of piperacillin (PIP) against beta-lactamase-negative ampicillin (AMP)-resistant (BLNAR) Haemophilus influenzae were compared with those of cefotaxime (CTX) and ceftriaxone (CRO), and the potency of PIP as therapy for meningitis caused by BLNAR is also discussed. PIP showed good activity (MIC at which 90% of strains are inhibited, 0.25 micro g/ml) against 69 BLNAR strains, and its activity was comparable to that of CRO and superior to that of CTX.

Prevalence of cefotaxime resistance in group B streptococcus isolates from Osaka, Japan.

Streptococcus agalactiae (group B streptococcus; GBS) organisms are a major cause of severe infections, including bacteremia and meningitis in newborns. According to previous reports, GBS organisms are uniformly sensitive to penicillin G (PCG). The susceptibility of 117 strains isolated at Yodogawa Christian Hospital in Osaka, Japan, in 2001 was examined with the WalkAway system, using currently valid National Committee for Clinical Laboratory Standards (NCCLS) interpretive criteria.

[M protein gene (emm) typing of Streptococcus pyogenes].

T-agglutination typing and M protein gene (emm) typing were determined on the isolates of Streptococcus pyogenes taken from patients in Osaka and neighboring districts during 1996-2000. A total of 701 isolates were classified to 15 kinds of T types and type untypable. In all isolates, T 12 was revealed as the most dominant serotype, followed by T1, T4 and T2.

[Streptococcus dysgalactiae subsp. equisimilis possessing Lancefield's group A antigen].

We isolated Streptococcus dysgalactiae subsp. equisimilis with Lancefield's group A antigen from a patient in Osaka City. This clinical isolate formed beta-hemolytic large colonies on sheep blood agar, and was catalase-negative gram-positive cocci carrying Lancefield's group A antigen.