New frequency weighting of hand-arm vibration.

A new frequency weighting method to assess hand-arm vibration exposure is proposed in this paper. The influence of frequency components was first estimated using the method of multivariate analysis, then a simple form of the weighting curve was introduced. Compared with the frequency weighting in the current ISO 5349-1 (2001), the proposed weighting can better explain the relationship between rates of symptoms and vibration exposure among vibrating tool users.

Purification and characterization of a novel cholesterol esterase from Pseudomonas aeruginosa, with its application to cleaning lipid-stained contact lenses.

With the aim of developing a new cholesterol esterase for eliminating lipids on used contact lenses, microorganisms were screened for the enzyme activity. A Pseudomonas aeruginosa isolated from soil was found to produce a desirable enzyme. The enzyme had an isoelectric point of 3.

A new type of aminoacyltransferase from Saccharothrix sp. AS-2 favorable for the synthesis of D-amino acid-containing peptides.

A unique enzyme with some properties favorable for the synthesis of D-amino acid-containing peptides has been purified from the culture broth of Saccharothrix sp. AS-2. The purification steps included ammonium sulfate fractionation, chromatographies on CM-Toyopearl 650M and ProtEx Butyl, and sucrose density-gradient isoelectric focusing.