Publications by authors named "Yoshinobu Terada"

Carotenoids, classified into carotenes and xanthophylls, are natural lipophilic pigments that are widely distributed in plants. Red paprika is unique in its high levels of various xanthophylls. Dietary paprika xanthophylls have been shown to reduce UV-induced photo damage by the strong antioxidant activity in the skin.

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Article Synopsis
  • Lutein is an important carotenoid used for plant photosynthesis and human health, particularly in preventing eye diseases.
  • Research on engineering the biosynthesis pathway for microbial production of lutein has been limited due to the complexity of the required genes from plants.
  • This study successfully integrated key carotenoid biosynthesis genes into the JM101 (DE3) strain, achieving a lutein yield of 11 mg/l, laying groundwork for future large-scale production using microorganisms.
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Capsanthin, a characteristic red carotenoid found in the fruits of red pepper (), is widely consumed as a food and a functional coloring additive. An enzyme catalyzing capsanthin synthesis was identified as capsanthin/capsorubin synthase (CCS) in the 1990s, but no microbial production of capsanthin has been reported. We report here the first successful attempt to biosynthesize capsanthin in by carotenoid-pathway engineering.

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We studied the nanostructure and physical properties of cellulose nanofiber-multi-walled carbon nanotube (CNF-MWNT) composite films prepared via MWNT aqueous dispersion using 4-O-methyl-α-d-glucuronoxylan as a MWNT dispersion aid. The composite film had high electrical conductivity (1.05S/cm), good mechanical properties (Young's modulus: 10.

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Amylomaltase from Thermus aquaticus catalyzes three types of transglycosylation reaction, as well as a weak hydrolytic reaction of alpha-1,4 glucan. From our previous study [Fujii et al., Appl.

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Amylomaltase from Thermus aquaticus catalyzes intramolecular transglycosylation of alpha-1,4 glucans to produce cyclic alpha-1,4 glucans (cycloamyloses) with degrees of polymerization of 22 and higher. Although the amylomaltase mainly catalyzes the transglycosylation reaction, it also has weak hydrolytic activity, which results in a reduction in the yield of the cycloamyloses. In order to obtain amylomaltase with less hydrolytic activity, random mutagenesis was perfromed for the enzyme gene.

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