A Novel Cellular Imaging Method Using Hemagglutinating Virus of Japan-Envelope (HVJ-E) Vector and Magnetic Particle Imaging.

The purpose of this study was to develop a novel cellular imaging method using the hemagglutinating virus of Japan-envelope (HVJ-E) vector and magnetic particle imaging (MPI). First, we determined the concentration of magnetic nanoparticles (MNPs) suitable for encapsulation into the HVJ-E vector (HVJ-MNPs). Colon-26 cells were labeled with HVJ-MNPs, MNPs conjugated with protamine (Pro-MNPs) or MNPs alone (Res-MNPs), and their labeling efficiencies were evaluated.

Magnetic Nanoparticles in Macrophages and Cancer Cells Exhibit Different Signal Behavior on Magnetic Particle Imaging.

Cell labeling with magnetic nanoparticles (MNPs) is a promising method of cell tracking. In particular, a novel quantitative tomography method called magnetic particle imaging (MPI) has the potential to estimate the number of successfully transplanted MNP-labeled cells, thereby helping predict clinical outcomes. However, the biological factors that shape the MPI signals of MNPs during cell labeling are not well understood.