Publications by authors named "Yoshikazu Wakizaka"
This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection.
View Article and Find Full Text PDFMicrofluidic dielectrophoresis (DEP) technology has been applied to many devices to perform label-free target cell separation. Cells separated by these devices are used in laboratories, mainly for medical research. The present study designed a microfluidic DEP device to fabricate a rapid and semiautomated cell separation system in conjunction with microscopy to enumerate the separated cells.
View Article and Find Full Text PDFBackground: PixeeMo™ is a compact instrument that enables bacterial cell counting using microfluidic chips instead of counting of colonies on culture media. Chips containing electrodes, based on fluid, electric filtering and sorting technology (FES), allow the selection of bacterial cells from other components in the sample. In the United States (US), surface water or ground water affected by surface water must be treated to reduce the total microbial load to less than 500 CFU/mL.
View Article and Find Full Text PDFSeparation of viable and nonviable animal cell using dielectrophoretic filter.
Biotechnol Prog
December 2010
Article Synopsis
- Selective separation of cells using dielectrophoresis (DEP) has limitations for large-scale applications due to inefficiencies near electrodes.
- A DEP filter was evaluated for its effectiveness in separating viable and nonviable animal cells, focusing on the impacts of AC electric field frequency and squared electric field intensity.
- Results showed a distinct frequency response for the different cell types, leading to an empirical equation for optimizing DEP separation conditions like voltage and flow rate.
Separation characteristics of animal cells using a dielectrophoretic filter.
Bioprocess Biosyst Eng
June 2010
Article Synopsis
- The study evaluated a wire-wire type dielectrophoretic (DEP) filter's ability to separate animal cells based on their activity.
- The results showed that cells retained in the DEP filter had a higher specific growth rate compared to those that permeated through it.
- Additionally, the separation process resulted in a maximum difference of about 20% in retention ratios between the two groups of separated cell suspensions.