Preparation and In Vitro Evaluation of W/O Nanoemulsions for the Skin Permeation of 5-Aminolevulinic Acid.

We investigated the skin permeation of 5-aminolevulinic acid (ALA) through Yucatan micropig full-thickness skin by using ALA-loaded W/O nanoemulsions composed of Span/Tween/ethanol (EtOH)/isopropyl palmitate (IPP)/10 wt% aqueous ALA solution. The nanoemulsions were prepared using Span 20/Tween 20 (S20/T20), Span 80/Tween 80 (S80/T80), and Span 20/Tween 80 (S20/T80) mixed surfactant systems. Based on the results of the phase diagram study and hydrodynamic diameter measurement of the nanoemulsions, we decided that the optimal weight ratio of Span/Tween/EtOH/IPP/10 wt% aqueous ALA solution in the nanoemulsion was 0.

5-Aminolevulinic acid-based photodynamic diagnosis for detection of urothelial carcinoma cells in bladder washing sediment suspension: A pilot study.

Background: Bladder cancer is a common malignant disease in developed countries. Early detection of malignancy is important using urine cytology. The 5-aminolevulinic acid (ALA)-based photodynamic diagnosis (ALA-PDD) has not been routinely applied in urine cytology analysis yet, although it has been well accepted for tumor lesion marking in cystoscopy.

[Detection of β-Lactamase Produced by Using High Performance Liquid Chromatography].

In order to develop a new method to detect β-lactamase, we determined degradation of β-lactam antibiotics, ampicillin sodium (ABPC) and cefotaxime sodium (CTX), by β-lactamase-producing () in terms of high performance liquid chromatography (HPLC). Using HPLC with an ODS column and an eluent composed of phosphate buffer and methanol, we could detect ABPC and CTX within 10 min. After cultured with , ABPC and CTX were degraded.

Accumulation of porphyrins in Propionibacterium acnes by 5-aminolevulinic acid and its esters.

We have investigated the accumulation of porphyrins in Propionibacterium acnes (P.acnes) by 5-aminolevulinic acid hydrochloride (ALA) and its esters, ALA methyl ester hydrochloride (mALA), ALA octyl ester hydrochloride (oALA), and ALA benzyl ester hydrochloride (bALA). From the fluorescence spectra of porphyrins accumulated in P.

Oxovanadium complexes with quinoline and pyridinone ligands: syntheses of the complexes and effect of alkyl chains on their apoptosis-inducing activity in leukemia cells.

Vanadium complexes with quinoline ligands (1b-g) and pyridinone ligands (2b-d) were synthesized, and the effect of the length and shape of alkyl chains on the antiproliferative activity toward U937 cells was studied. For the synthesis of the vanadium complexes, quinoline and pyridinone ligands were prepared and then treated with VOSO(4) or VO(acac)(2). The vanadyl(IV) complexes were characterized by IR, ESR, and UV-vis spectroscopy and elemental analyses.

Synthesis of oxovanadium complexes and their apoptosis-inducing activity in leukemia cells.

Vanadium complexes with different ligands were synthesized and evaluated for antiproliferative activity on U937 cells. The alkyl chain length of the ligands affected the antiproliferative activity, and two complexes-3b and 4-exhibited strong activities with IC(50) values of 6.02 and 3.

Enhancement in skin permeation of 5-aminolevulinic acid using l-menthol and its derivatives.

Enhancing effect of l-menthol and its derivatives, l-menthyl formate, l-menthyl acetate, and l-menthyl propionate, on skin permeation of 5-aminolevulinic acid (ALA) through Yucatan micropig full-thickness skin was investigated using a Franz-type diffusion cell. ALA solutions were prepared using ethanol-water mixed solvents with l-menthol or the derivative. Skin permeation coefficients (Kp) of ALA with more than 3.

Oxidation decomposition of unsaturated fatty acids by singlet oxygen in phospholipid bilayer membranes.

Oxidation decomposition of unsaturated fatty acids with singlet oxygen generated from a photosensitizing agent was investigated in liposome bilayer membranes under a light irradiation condition. The liposome of which the bilayer membrane was composed of L-alpha-dipalmitoylphosphatidylcholine (DPPC), protoporphyrin IX (PpIX), and an unsaturated fatty acid (oleic acid, linoleic acid, alpha-linolenic acid, or arachidonic acid) were prepared with Bangham's method. In irradiating the liposome dispersion with light ranged from 550 to 750 nm, the unsaturated fatty acid was decomposed through an oxidation reaction with singlet oxygen.

Immobilization and enzymatic activity of glucose oxidase on polystyrene surface modified with ozone aeration and UV irradiation in distilled water and/or aqueous ammonia solution.

Adsorption condition and enzymatic activity of glucose oxidase (GOD) on polystyrene (PS) film surfaces modified with ozone aeration and UV irradiation (O3/UV) treatment were investigated. The total amount of GOD immobilized on the PS film modified with the O3/UV treatment in distilled water (PS-W film) was approximately twice as large as that on the film treated in an aqueous ammonia solution (PS-A film), whereas the specific activity of GOD on the PS-A film was four times higher than that on the PS-W film. In contrast, no enzymatic activity of GOD on the non-treated PS film was observed because of irreversible denaturation of the adsorbed GOD.

Delta-aminolevulinic acid-based photodynamic therapy for acne on the body.

Intractable acne on the body was clinically treated with photodynamic therapy (PDT) combining oral administration of delta-aminolevulinic acid (ALA) with polychromatic visible light from a metal halide lamp. The total number of body acne patients was 51. A 10 mg/kg B.

Sintering behavior and apatite formation of diopside prepared by coprecipitation process.

Sintering behavior and bioactivity of diopside, CaMgSi(2)O(6), prepared by a coprecipitation process were examined for its biomedical applicability. As-prepared powder was synthesized by adding aqueous ammonia to an ethanol solution containing Ca(NO(3))(2).4H(2)O, Mg(NO(3))(2).