Preliminary validation of the self-report measure assessing experiences of negative independent and dependent event frequency in Japanese university students.

Background: In the past, different stress generation studies have used self-report measures comprising different items to assess each category of negative events. Moreover, the validity of these scales has not been adequately investigated. Therefore, we developed a self-report measure dedicated to assessing experiences of negative interpersonal dependent events, negative non-interpersonal dependent events, and negative independent events in university students, which was named the Negative Independent/Dependent Events Scale.

[Effects of internal working models of attachment on cognition about facial expressions].

This study examined the effects of internal working models of attachment on cognition about emotion in facial expressions. Ninety-five university students participated in a survey and an experiment. The results revealed that (a) effects of internal working models of attachment emerged when there were discrepancies between facial expressions and the emotions that should be rated, (b) "anxiety" did not affect the rating scores or reaction times, and (c) participants who scored high in "avoidance" needed more time to judge the absence of emotions in facial expressions.

[Validity of hypotheses about the father/mother interpretation of Rorschach cards: tested by using a card selecting method].

This study tests the validity of hypotheses about the interpretations of Rorschach father/mother cards by having subjects select the cards. The results showed that regardless of the subject's gender, Card IV is far more often selected as the father card. However Card VII is not more often chosen as the mother card, if anything, the Color Cards are usually selected.

[Is setting high personal standard really adaptive?: adult attachment style is a moderator].

Perfectionism is usually reported to have adverse affects on psychological health, but recent multidimensional research indicated that a "tendency to set high personal standards" is weakly correlated with reduced depressiveness. The present study categorized subjects using a cluster analysis with factors closely related to psychological health such as adult attachment styles and perfectionism, and examined each factor's contributions to psychological health. Among subjects with high personal standards, the study found a group with a secure attachment style who are psychologically adaptive and another group with a non-secure attachment style who are maladaptive.

Cell-based and biochemical structure-activity analyses of analogs of the microtubule stabilizer dictyostatin.

Compounds that bind to microtubules (MTs) and alter their dynamics are highly sought as a result of the clinical success of paclitaxel and docetaxel. The naturally occurring compound (-)-dictyostatin binds to MTs, causes cell cycle arrest in G(2)/M at nanomolar concentrations, and retains antiproliferative activity in paclitaxel-resistant cell lines, making dictyostatin an attractive candidate for development as an antineoplastic agent. In this study, we examined a series of dictyostatin analogs to probe biological and biochemical structure-activity relationships.

Fluorous mixture synthesis of (-)-dictyostatin and three stereoisomers.

[reaction: see text] A mixture of four stereoisomers whose configurations are encoded by fluorous silyl protecting groups has been prepared and converted over 22 steps to a mixture of protected dictyostatins. Demixing by fluorous HPLC followed by removal of the fluorous protecting groups (detagging) provides dictyostatin and three C6,C7 stereoisomers. Biological evaluation showed that the monoepimers of the natural product retained highly potent activity.

Tubulin assembly, taxoid site binding, and cellular effects of the microtubule-stabilizing agent dictyostatin.

(-)-Dictyostatin is a sponge-derived, 22-member macrolactone natural product shown to cause cells to accumulate in the G2/M phase of the cell cycle, with changes in intracellular microtubules analogous to those observed with paclitaxel treatment. Dictyostatin also induces assembly of purified tubulin more rapidly than does paclitaxel, and nearly as vigorously as does dictyostatin's close structural congener, (+)-discodermolide (Isbrucker et al. (2003), Biochem.