Epidemiology of bovine brucellosis by a combination of rose bengal test and indirect ELISA in the five districts of Uganda.

A serological survey on bovine brucellosis was carried out 3 times between 2007 and 2009 in 3 districts (Kiboga, Mpigi and Kiruhura) in western Uganda and 2 (Kumi and Mbale) in the east employing the rose bengal test (RBT) for infected-herd screening and an indirect ELISA (iELISA) for testing the serostatus of individual animals. The animal prevalence was significantly higher in the 3 districts of the west (mean 21.5% in 2009) compared with the 2 districts (mean 3.

Reactivity of anti-Nipah virus monoclonal antibodies to formalin-fixed, paraffin-embedded lung tissues from experimental Nipah and Hendra virus infections.

The immunohistochemical reactivity of seven clones of mouse monoclonal antibodies raised to Nipah virus antigens were investigated using formalin-fixed, paraffin embedded porcine and equine lung tissues from experimental Nipah and Hendra virus infection, respectively. Either microwave irradiation or enzymatic digestion effectively unmasked the viral antigens in formalin-fixed, paraffin-embedded tissue sections. Four clones showed positive reaction to both Nipah virus-infected porcine lung tissue and Hendra virus-infected equine lung tissue.

A solid-phase blocking ELISA for detection of antibodies to Nipah virus.

A monoclonal antibody (MAb) based solid-phase blocking ELISA was developed for detection of antibodies to Nipah virus. The ELISA was designed to detect remaining antigens on the plate with anti-Nipah MAb conjugate after the reaction with sample serum, and enabled simple procedure, detection of neutralizing antibody to Nipah virus, and application of samples from different animal species. Forty of 200 swine reference sera examined were positive by the ELISA, of which thirty seven were found positive by serum neutralization test.

Seroepidemiology of neosporosis in dairy cattle in Uruguay.

A seroepidemiological survey of Neospora caninum was done in a dairy farm in Uruguay employing indirect fluorescent antibody test (IFAT). Sera from 217 cattle (155 cows, 31 heifers and 31 calves) were tested for N. caninum antibodies in July 2000 and it was found that abortions were significantly associated with the seropositivity of the cows by chi(2)-test (P = 0.

Production and characterization of monoclonal antibodies against formalin-inactivated Nipah virus isolated from the lungs of a pig.

Eight clones of monoclonal antibodies (Mabs) to Nipah virus (NV) were produced against formalin-inactivated NV antigens. They reacted positive by indirect immunofluorescent antibody test, and one of them also demonstrated virus neutralizing activity. They were classified into six different types based on their biological properties.