Development of a novel cell-based assay to evaluate the malignant potential of cancer in vitro.

Background: Metastasis is a leading cause of cancer death. To evaluate the complex metastatic process in vitro, an attempt was made to develop a cell-based assay (Can kit) that could evaluate the late stages of metastasis.

Materials And Methods: Two membrane chambers were set up of which the upper membrane chamber with 8 mum pores was covered with normal cell layers.

New method for separation of subpopulations from a heterogeneous colon cancer cell line.

Background: To understand the heterogeneity of human colon cancers, a new method to separate cancer subpopulations was developed.

Materials And Methods: Cells from a human colon cancer cell line, DLD-1, were seeded on an 8 microm pore membrane. After six hours, the cells which remained beneath the membrane as well as the cells which dropped onto the 24-well plate were collected.