Dynamic Metabolome Analysis Reveals the Metabolic Fate of Medium-Chain Fatty Acids in AML12 Cells.

Several studies in hepatocyte cell lines reported that medium-chain fatty acids (MCFAs) with 6-12 carbons showed different metabolic properties from long-chain fatty acids (LCFAs). However, these studies reported unclear effects of different fatty acid molecules on hepatocyte metabolism. This study is aimed to capture the metabolic kinetics of MCFA assimilation in AML12 cells treated with octanoic acid (FA 8:0), decanoic acid (FA 10:0), or lauric acid (FA12:0) [LCFA; oleic acid (FA 18:1)] metabolic profiling and dynamic metabolome analysis with C-labeling.

Dietary soybean phospholipids increase the oxidative stability of lipids extracted from fish fillets.

We previously reported that the feeding of soybean phospholipids to fish increased the storage stability of n-3 polyunsaturated fatty acids (PUFA)-rich fish fillets. In this study, we examined the storage stability of lipids extracted from fish fed a diet containing soybean phospholipids and fish oil. Rainbow trout were divided into two groups, and were fed an either 2.

Effect of life-long dietary n-6/n-3 fatty acid ratio on life span, serum lipids and serum glucose in Wistar rats.

Types of dietary lipid affect the life span of rats. In this study, we investigated the influence of the life-long dietary n-6/n-3 ratio on life span and serum lipid and glucose levels. A semi-purified diet adjusted to a constant saturated : monounsaturated : polyunsaturated fatty acid ratio and an n-6/n-3 ratio of 1 (R1), 4 (R4) or 16 (R16) was fed to rats (n=33) from 4 wk of age until death.

Characterization of glutamate decarboxylase mediating gamma-amino butyric acid increase in the early germination stage of soybean (Glycine max [L.] Merr).

Glutamate decarboxylase (GAD) is an enzyme that catalyzes the production of gamma-amino butyric acid (GABA) from glutamate through a decarboxylation reaction. A full-length cDNA encoding glutamate decarboxylase (GmGAD1) was isolated from germinating soybean seeds (Glycine max [L.] Merr.

Alpha-linolenic acid-rich flaxseed oil ingestion increases plasma adiponectin level in rats.

Adiponectin, an adipose-specific secretory protein, exhibits antidiabetic and antiatherogenic properties. The effect of alpha-linolenic acid (ALA) on adiponectin has not been revealed. ALA is included abundantly in vegetable oils such as flaxseed oil.

Effect of dietary n-6/n-3 ratio on liver n-6/n-3 ratio and peroxisomal beta-oxidation activity in rats.

The optimal dietary n-6/n-3 ratio has not been fully elucidated. To investigate the influence of the dietary n-6/n-3 ratio on this ratio in the body and on liver beta-oxidation peroxisomal activity, rats were fed diets containing fat at an n-6/n-3 ratio of 1 to 16 for 4 weeks. To investigate whether elevation of the liver peroxisomal beta-oxidation activity increases the n-6/n-3 ratio in the body, rats were fed a diet containing a peroxisome-activating agent, bezafibrate, for 2 weeks, and its influence on the liver n-6/n-3 ratio was examined.

Effect of dietary lard containing higher alpha-linolenic acid on plasma triacylglycerol in rats.

It is well known that the consumption of n-3 polyunsaturated fatty acid (PUFA) decreases the plasma triacylglycerol (TAG) level. The technology of elevating the content of n-3 PUFAs in pig meat has already reached a practical level. In this study, the effects of dietary lard containing higher alpha-linolenic acid (LNA) on plasma TAG were compared with those of normal lard in rats.

Antihypertensive effect and safety of dietary alpha-linolenic acid in subjects with high-normal blood pressure and mild hypertension.

We investigated the antihypertensive effect and safety of alpha-linolenic acid (ALA) in human subjects. In Experiment 1, subjects with high-normal blood pressure and mild hypertension ingested bread containing 14 g of common blended oil (control oil) or ALA-enriched oil for 12 weeks. The test oil contained 2.