Susceptibility to SARS-CoV-2 and MERS-CoV in Beagle Dogs.

The coronavirus disease 19 (COVID-19) pandemic, caused by the severe acute respiratory syndrome, coronavirus 2 (SARS-CoV-2), has resulted in unprecedented challenges to healthcare worldwide. In particular, the anthroponotic transmission of human coronaviruses has become a common concern among pet owners. Here, we experimentally inoculated beagle dogs with SARS-CoV-2 or Middle East respiratory syndrome (MERS-CoV) to compare their susceptibility to and the pathogenicity of these viruses.

Protective efficacy of attenuated Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of in goats.

Background: Attenuated strain can be used as a vector to transport immunogens to the host antigen-binding sites.

Objectives: The study aimed to determine the protective efficacy of attenuated strain expressing highly conserved immunogens in goats.

Methods: Goats were vaccinated with vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination.

Vaccination of goats with a combination vector expressing four antigens (BLS, PrpA, Omp19, and SOD) confers protection against infection.

is an intracellular pathogen with a cellular infection mechanism similar to that of , making it a suitable choice for use in an anti- immune boost system. This study explores the efficacy of a Typhimurium delivery-based combination vaccine for four heterologous antigens ( lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 10 colony-forming unit (CFU)/mL (Group B) and 5 × 10 CFU/mL (Group C) and challenged the inoculations with virulent at 6 weeks post-immunization.