Fluorescent polymer nanoparticle for selective sensing of intracellular hydrogen peroxide.

Fluorescent boronate-modified polyacrylonitrile (BPAN) nanoparticles of 50 nm diameter were fabricated for use as a selective H(2)O(2) sensor. The fluorescence intensity changed and an emission peak shifted when BPAN nanoparticles selectively interacted with H(2)O(2), relative to other reactive oxygen species (ROS). The BPAN nanoparticles undergo photoinduced electron transfer (PET) between a Schiff base moiety and boronate, which enhances the fluorescence and makes the nanoparticles suitable for selective ROS recognition.

Fluorescent europium-modified polymer nanoparticles for rapid and sensitive anthrax sensors.

Novel fluorescent polyacrylonitrile nanoparticles were synthesized by microemulsion polymerization and Schiff base modification. By further modification with europium, the polyacrylonitrile nanoparticles could be used as a highly sensitive and rapid sensor for Bacillus anthracis spore detection in aqueous solution. The europium-modified polyacrylonitrile nanoparticles were readily combined with dipicolinic acid as a unique biomarker of B.

Cellular uptake, cytotoxicity, and ROS generation with silica/conducting polymer core/shell nanospheres.

The cellular response to conducting polymer (CP) nanospheres with similar physical properties was evaluated by in vitro cellular uptake and cytotoxicity in mouse macrophage RAW 264.7 and rat pheochromocytoma PC-12 cells. Four different CPs (polythiophene, poly(3,4-ethylenedioxythiophene), polyaniline, and polypyrrole) were deposited onto silica nanoparticles with a diameter of ca.

Cytotoxicity of, and innate immune response to, size-controlled polypyrrole nanoparticles in mammalian cells.

Monodisperse polypyrrole (PPy) nanoparticles with five different diameters (20, 40, 60, 80, and 100 nm) were fabricated via chemical oxidation polymerization in order to evaluate size-dependent cytotoxicity. The cellular uptake of PPy nanoparticles in human lung fibroblasts (IMR90) and mouse alveolar macrophages (J774A.1) was observed by transmission electron microscopy.

Cellular uptake, cytotoxicity, and innate immune response of silica-titania hollow nanoparticles based on size and surface functionality.

Silica-titania hollow nanoparticles (HNPs) with uniform diameters of 25, 50, 75, 100, and 125 nm were fabricated by dissolution and redeposition method in order to evaluate size dependent cellular response. Surface-modified HNPs with cationic, anionic, and neutral functional group were prepared by silane treatment. We systematically investigated cellular internalization, toxicity, and innate immune response of HNPs in human breast cancer (SK-BR-3) and mouse alveolar macrophage (J774A.