Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis.

The Mycobacterium avium complex (MAC) is a group of closely related nontuberculous mycobacteria that can cause various diseases in humans. In this study, genome sequencing, comprehensive genomic analysis, and antimicrobial susceptibility testing of 66 MAC clinical isolates from King Chulalongkorn Memorial Hospital, Bangkok, Thailand were carried out. Whole-genome average nucleotide identity (ANI) revealed the MAC species distribution, comprising 54 (81.

CRISPR Interference-Mediated Silencing of the Gene in and Its Impact on Antimicrobial Susceptibility.

Background: The discovery of novel therapeutic agents, especially those targeting mycobacterial membrane protein large 3 (), has shown promise. In this study, the CRISPR interference- nuclease-deactivated Cas9 (CRISPRi-dCas9) system was utilized to suppress expression in , and its impacts on susceptibility to antimicrobial agents were evaluated.

Methods: The repression of the gene was confirmed by RT-qPCR.