Zero-waste multistage utilization of dandelion root.

Introduction: In the fragrance and perfume industry, the controlled release of fragrances are crucial factors that contribute to consumer appeal and product quality enhancement. In this study, various aromatic active substances were extracted from dandelion root (DR), which was subsequently calcined to produce high-performance porous biochar material.

Methods: The dandelion root biochar (DRB) material was identified as promising adsorbents for the controlled release of fragrances.

Naked-eye detection of plant viral disease using polymerase chain reaction amplification and DNAzyme.

Plant viral diseases can seriously affect the yield and quality of crops. In this work, a convenient and highly sensitive biosensor for the visual detection of plant viral disease is proposed by the PCR-induced generation of DNAzyme. In the absence of nucleic acid for a target plant virus, the primers prohibited the production of G-quadruplex by forming a hairpin structure.

[Expression and significance of hTERT, c-myc and Ki-67 in hepatocellular carcinoma].

Aim: To study the correlation of expression of hTERT, c-myc and Ki-67 with the clinical pathological feature of hepatocellular carcinoma and explore the relationship among the three factors.

Methods: The expressions of hTERT, c-myc and Ki-67 in 37 cancer tissues and 5 normal tissues were assessed by immunohistochemical method.

Results: The positive rates of hTERT, c-myc and Ki-67 in the cancer tissue were higher than those in normal tissues (P<0.

[Expression of GFP gene in mouse early embryo produced by sperm-mediated gene transfer].

The efficiency of the exogenous DNA transfecting mouse sperm was studied by the DIG end labeled and immunohistochemistry technology. The results suggested that: the efficiency of transfecting positive rate of individual mouse sperm was distinct difference (P < 0.01), and the average rate was 13%.

[Nuclear transfer of goat somatic cells transgenic for human lactoferrin].

Transgenic animal mammary gland bioreactors are being used to produce recombinant proteins with appropriate post-translational modifications, and nuclear transfer of transgenic somatic cells is a more powerful method to produce mammary gland bioreactor. Here we describe efficient gene transfer and nuclear transfer in goat somatic cells. Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin (LF) gene cDNA into exon 2 of the milk goat beta-casein gene, and the endogenous start condon was replaced by that of human LF gene.