Publications by authors named "Yongsong Yue"
Background: Factors predicting peripheral blood total HIV-1 DNA size in chronically infected patients with successfully suppressed viremia remain unclear. Prognostic power of such factors are of clinical significance for making clinical decisions.
Methods: Two sets of study populations were included: 490 China AIDS Clinical Trial (CACT) participants (Training cohort, followed up for 144 to 288 weeks) and 117 outpatients from Peking Union Medical College Hospital (PUMCH) (Validation cohort, followed up for more than 96 weeks).
Article Synopsis
- A new efficient and reproducible method was developed for assessing total HIV-1 DNA levels using frozen whole blood and PBMC samples to improve consistency in sample extraction and detection.
- The study involved 44 patients, predominantly male (42 males, 2 females), with various treatment histories, indicating a median viral load and immune cell counts that were consistent across both sample types.
- The findings suggest that whole blood samples can reliably replace PBMC samples for measuring total HIV-1 DNA in clinical settings, making the testing process simpler and more efficient.
Background: The impact of HIV-1 subtype (CRF01_AE and non-CRF01_AE) on HIV-1 DNA levels in HIV-1 chronically infected patients with suppressive antiretroviral therapy (ART) remains poorly understood. To evaluate the correlation of HIV-1 subtype with DNA level, and identify baseline predictors of HIV-1 DNA decay.
Methods: ART-naïve HIV-1-infected patients from two large multi-center studies in China were classified into CRF01_AE and non-CRF01_AE subtype groups.
Background: The effect of ART initiation time on HIV-1 DNA reservoir in chronically infected individuals is not well understood. Determining the potential influencing factors associated with a low HIV-1 DNA level in chronic infection is an important step toward drug-free control.
Methods: A prospective study included 444 chronically HIV-infected adults was performed.
Background: The HIV-1 DNA reservoir is an important marker that reflects viro-immunological status and can be affected by multiple viral or cellular factors. Determining the potential factors associated with the size of the HIV-1 DNA reservoir benefits the surveillance of disease progression and antiretroviral treatments.
Methods: We conducted a case control study to explore the factors that may affect the level of HIV-1 DNA.
[Long non-coding RNA Gm15577 is involved in mouse cerebellar neurogenesis].
Zhonghua Bing Li Xue Za Zhi
July 2015
Objective: To identify novel lncRNAs involved in cerebellar neurogenesis using neuronal specific Nbs1-deficient (Nbs1(CNS-del)) mouse model.
Methods: Microarray analysis was performed to identify differentially expressed lncRNAs between Nbs1(CNS-ctr) and Nbs1(CNS-del) mice. Expression profiles of lncRNA Gm15577 and coding gene Negr1 in mice, primary cerebellar culture and cell lines were measured using RT-qPCR.