Molecular detection and phylogeny of Anaplasma spp. closely related to Anaplasma phagocytophilum in small ruminants from China.

The genus Anaplasma comprises eight bacterial species that are obligate intracellular pathogens that affect human and animal health. The zoonotic species A. phagocytophilum is the causative agent of tick-borne fever in ruminants, and of granulocytic anaplasmosis in horses, dogs, and humans.

The Novel Zoonotic Pathogen, , Infects Human Erythrocytes, HL-60, and TF-1 Cells .

, a species of the family Anaplasmataceae, is zoonotic tick-borne obligate intracellular bacteria. There have been no reports of human infection with this pathogen since 2015. Therefore, the zoonotic characteristics of need to be further studied.

The first detection of , an emerging zoonotic sp., in erythrocytes.

An emerging infectious disease caused by "" was reported in a 2015 survey of 477 hospital patients with a tick-bite history in China. However, the morphological characteristics and parasitic location of this pathogen are still unclear, and the pathogen has not been officially classified as a member of the genus . -positive blood samples were collected, blood cells separated, and DNA of whole blood cells, erythrocytes, and leukocytes extracted.

A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Species in Field Blood Samples.

The genus (Rickettsiales: Anaplasmataceae), which includes the species , , , and , is responsible for a wide variety of infections in both human and veterinary health worldwide. Multiple infections with these four pathogens have been reported in many cases. We introduce a novel multiplex PCR for the simultaneous detection of , , , and , based on species-specific primers against the ( and ), (), and 16S rRNA () genes.

Dogs as New Hosts for the Emerging Zoonotic Pathogen in China.

is an emerging zoonotic tick-borne pathogen with a broad host range, including many mammals. Dogs have close physical interactions with humans and regular contact with the external environment. Moreover, they have been previously reported to be hosts of , and .

Duplex TaqMan real-time PCR assay for simultaneous detection and quantification of Anaplasma capra and Anaplasma phagocytophilum infection.

Anaplasma capra and A. phagocytophilum, two species of the family Anaplasmataceae, are zoonotic tick-borne obligate intracellular bacteria affecting wild and domestic ruminants, dogs, cats, horses and humans. A.

Rapid and sensitive detection of Anaplasma phagocytophilum using a newly developed recombinase polymerase amplification assay.

Anaplasma phagocytophilum, the bacterial pathogen responsible for tick-borne fever and human granulocytic anaplasmosis, can seriously affect the health of humans and a wide range of other mammals. In this study, we developed a recombinase polymerase amplification (RPA) assay to detect A. phagocytophilum in clinical samples.

Detection and Phylogenetic Characterization of : An Emerging Pathogen in Sheep and Goats in China.

is an emerging pathogen, which can infect ruminants and humans. This study was conducted to determine the occurrence of in the blood samples of sheep and goats in China. Using nested polymerase chain reaction (nested-PCR) targeting the gene and conventional PCR targeting the heat shock protein () gene and the major surface protein4 gene (), was detected in 129 (8.