Regional antimicrobial resistance gene flow among the One Health sectors in China.

Background: Antimicrobial resistance poses a significant threat to global health, with its spread intricately linked across human, animal, and environmental sectors. Revealing the antimicrobial resistance gene (ARG) flow among the One Health sectors is essential for better control of antimicrobial resistance.

Results: In this study, we investigated regional ARG transmission among humans, food, and the environment in Dengfeng, Henan Province, China by combining large-scale metagenomic sequencing with culturing of resistant bacterial isolates in 592 samples.

Preparation of sulfonic acid functionalized metal organic frameworks and their application in the online solid phase extraction of parabens and sulfonamides in pre-cooked foods.

Unlabelled: Mixed-mode sorbents exhibit two or more primary retention mechanisms, which can enhance the selectivity and capacity of the extraction process in a single step. In this study, a facile approach was proposed to prepare functionalized metal-organic frameworks (MOFs) by post-synthetic oxidation. The composites could be varied independently for each processing step, resulting in four frameworks to meet different sample pretreatment requirements.

Benzotrithiophene-based covalent organic frameworks for sensitive fluorescence detection and efficient removal of Ag from drinking water.

The simultaneous detection and removal of Ag from drinking water was crucial for preventing human health, while it was also extremely challenging due to bifunctional materials that combine both Ag adsorption and detection functions rarely being explored. In this study, a benzotrithiophene-based covalent organic framework (TAPA-BTT) was synthesized and applied to detect and remove Ag. TAPA-BTT exhibited high crystallinity, a large specific surface area, and good thermal stability.

Hydroxylated magnetic microporous organic network for efficient magnetic solid phase extraction of trace triazine herbicides.

Here we covalently constructed abundant long-chain hydroxyl groups-functionalized magnetic microporous organic networks (MMON-2OH) for detection of eight Triazine herbicides (THs) in honey and water samples. MMON-2OH owned a high surface area (287.86 m²/g), enhanced water compatibility, and increased exposure of long-chain hydroxyl groups, which significantly improved enrichment capacity for THs.

A Milk Extracellular Vesicle-Based Nanoplatform Enhances Combination Therapy Against Multidrug-Resistant Bacterial Infections.

The increasing occurrence of infections caused by multidrug-resistant (MDR) bacteria drives the need for new antibacterial drugs. Due to the current lack of antibiotic discovery and development, new strategies to fight MDR bacteria are urgently needed. Efforts to develop new antibiotic adjuvants to increase the effectiveness of existing antibiotics and design delivery systems are essential to address this issue.

Effect of UV-C irradiation treatment on mycotoxins production in Fusarium species inoculated wheat seeds during wheat germination.

Wheat is a most important food crop worldwide. Wheat is reported to be susceptible to a variety of fungi, which could induce huge economic losses and the contamination of potential mycotoxins could bring serious toxic effects. In this work, UV-C irradiation treatment on Fusarium infected wheat seeds during germination was investigated.

Amorphous cassava starch/spirulina protein mixtures stabilized Pickering emulsions: Preparation and stability.

This study explored stabilized emulsions using cassava starch (CS) and spirulina protein (SP) mixtures, targeting microbial proteins as potential replacements for animal proteins in food stability applications. The final viscosity and enthalpy change of the CS/SP mixtures decreased from 3.78 to 1.

Modifying effects of prenatal exposure to rare earth elements on birth outcomes through maternal thyroid function in early pregnancy.

The potential health effects of exposure to rare earth elements (REEs) remain largely unexplored. This prospective cohort study aimed to elucidate the association between early pregnancy REE exposure and maternal thyroid function, as well as neonatal birth outcomes, in a cohort of pregnant women in Beijing, China. Additionally, the study explored the mediating role of thyroid homeostasis in the effects of REE exposure.

High carriage and possible hidden spread of multidrug-resistant Salmonella among asymptomatic workers in Yulin, China.

Food workers have frequent contact with unprocessed foods, but their carriage of Salmonella and potential influence on public health have not been comprehensively assessed. We investigated Salmonella carriage among food workers compared with non-food workers based on occupational health screening of 260,315 asymptomatic workers over an 8-year surveillance period in Yulin, China. We confirmed that healthy carriers serve as natural reservoirs for Salmonella, with higher carriage rates in food workers than non-food workers.

The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment.

Seafood consumption is the major source of total Hg (tHg) and methyl mercury (MeHg) for humans. Lack of broad-representative bio-accessibility of mercury species makes accurate assessment on health risk of seafood's mercury impossible. Herein, the concentrations and in vitro bio-accessibilities of mercury species in 93 seafood samples with 71 different species were extensively investigated.

Simple and rapid determination of 3-monochloropropane-1,2-diol in food contact papers based on polydopamine-polyethyleneimine copolymerization.

The aim of this work was to develop a fluorescence method based on the polydopamine-polyethyleneimine (PDA-PEI) copolymerization, which was subsequently applied for the determination of 3-monochloropropane-1,2-diol (3-MCPD) in food contact papers (FCMs). PEI could provide an alkaline environment and then react with dopamine (DA) to produce copolymers by Michael addition and Schiff-base reactions. This copolymer has a strong fluorescence emission at 527 nm.

Nanopore-assisted ELISA for ultrasensitive, portable, and on-site detection of ricin.

Effective detection technologies in food safety with the merits of portable and on-site detection potential are always in pressing demand. Herein, we developed a nanopore-assisted Enzyme-linked immunosorbent assay (NELISA) platform, which innovatively introduced hairpin DNA (HP DNA) probes as reaction substrates. This innovation of substrates effectively avoided the inherent limitations of colorimetric signals (i.

Interfacial structure modification and enhanced emulsification stability of microalgae protein through interaction with anionic polysaccharides.

Microalgae protein (MP) have emerged as a focal point of research within food processing due to its nutritional value and foaming properties. However, its isoelectric point around pH 4 leads to it susceptible to collision, binding, and precipitation. Additionally, MP has poor emulsification properties and only shows stability under strongly alkaline conditions.

Epidemiological and genomic analysis revealed the significant role of flies in dissemination of carbapenem-resistant Enterobacteriaceae (CRE) in China.

While the role of flies as potential vectors for bacterial transmission is well recognized, the epidemiological features and genomic characteristics of associated antimicrobial-resistant strains remain underexplored. This study conducted a nationwide surveillance including 3689 flies and 838 isolates from sixteen human communities (HCs) and eight animal farms (AFs) across 21 provinces in China. Our findings revealed high carriage rates of carbapenem-resistant Enterobacteriaceae (CRE) in flies, with 27.

Integration of bifunctional silver dendrite membranes with surface-enhanced Raman scattering for sensitive detection of polystyrene microplastics in aquatic environments.

Microplastics (MPs) are emerging environmental pollutants that are present in aquatic environments and accumulate within the food chain, posing significant threats to human health. Over 8 million tons of MPs enter these ecosystems annually. However, existing rapid qualitative and quantitative analytical methods for trace MPs are limited, hindering comprehensive research on their impact in water environments.

A nanopore-based label-free CRISPR/Cas12a system for portable and ultrasensitive detection of zearalenone.

Background: Food safety has become a serious global concern. Therefore, there is a need for effective detection technologies in this field. Currently, the development of effective on-site detection techniques is extremely important for food safety.

Nanopore-Based Biomimetic ssDNA Reporters for Multiplexed Detection of Meat Adulteration.

Meat adulteration detection is crucial for ensuring food safety, protecting consumer rights, and maintaining market integrity. However, the current methods face challenges in achieving multiplexed detection through efficient signal conversion and output. This study introduces a nanopore-based approach for the simultaneous detection of multiple meat adulteration.

A Clickase-Mediated Immunoassay Based on Nanopore and Bionic Signal Labels for Ultrasensitive, Portable, and On-Site Detection of Ricin.

It is of particular importance to develop an effective method that possesses several merits simultaneously of rapid, ultrasensitive, portable, and on-site detection potential for food safety detection. Herein, we propose a clickase-mediated immunoassay based on nanopore and bionic signal labels for the detection of ricin. The introduction of Cu/Cys clickase and nanopore simultaneously effectively addressed the inherent limitations of natural enzymes and colorimetric signal output, respectively.

Effect of Chlorella pyrenoidosa and Spirulina platensis powder on the physicochemical, structural, and rheological properties of rice starch: A comparative study.

The effect of Chlorella pyrenoidosa (CP) and Spirulina platensis (SP) at concentrations of 0 %-12 % on the properties of rice starch (RS) was investigated. Compared with pure RS, the addition of CP and SP powder decreased the viscosity, increased the gelatinization temperature, and promoted the retrogradation of RS gel. However, when CP was added at 12 % and SP at 8 %, retrogradation inhibition was reduced.