Metagenomics insights into the profiles of antibiotic resistome in combined sewage overflows from reads to metagenome assembly genomes.

Combined sewage overflows (CSOs) have become an important source of antibiotic resistance genes (ARGs) in the environment, while the distribution and dynamics of antibiotic resistome in the CSOs events have not been well understood. This study deciphered the profiles of antibiotic resitome in the CSOs based on metagenomics analysis from reads to metagenome assembly genomes (MAGs), and the dynamical changes of ARGs were clarified through continuous monitoring of the CSO event. Results showed that antibiotic inactivation was the dominant resistance mechanism, and sulfonamide, aminoglycoside along with multidrug resistance were the dominant antibiotic resistance types.

Effect of rainfall characteristics on the sewer sediment, hydrograph, and pollutant discharge of combined sewer overflow.

Significant management needs raised in urban sewer system to facilitate urban resilience to rainstorm. The work investigated the effects of temporal evolution of rainfall on hydrograph and pollutant discharge of CSO over an intensive observation period of 12 months, with special attention to differences in temporal scale for supporting management decision making. The characteristics of rainfall in different temporal scales helped overflow-risk identification and assessment.

Fates of intracellular and extracellular antibiotic resistance genes and microbial community structures in typical swine wastewater treatment processes.

Swine wastewater is an important reservoir of spread antibiotic resistance to the environment. Intra- and extracellular antibiotic resistance genes (iARGs and eARGs) were quantified during two typical swine wastewater treatment processes including a sequencing membrane bioreactor (SMBR) at pilot-scale and anaerobic-anoxic-oxic (AO) at full-scale. The concentrations of iARGs and eARGs in raw wastewater were 3.