Nomograms to predict lung metastasis in malignant primary osseous spinal neoplasms and cancer-specific survival in lung metastasis subgroup.

Purpose: To construct and validate nomograms for predicting lung metastasis probability in patients with malignant primary osseous spinal neoplasms (MPOSN) at initial diagnosis and predicting cancer-specific survival (CSS) in the lung metastasis subgroup.

Methods: A total of 1,298 patients with spinal primary osteosarcoma, chondrosarcoma, Ewing sarcoma, and chordoma were retrospectively collected. Least absolute shrinkage and selection operator (LASSO) and multivariate logistic analysis were used to identify the predictors for lung metastasis.

Genomic signatures associated with recurrent scale loss in cyprinid fish.

Scale morphology represents a fundamental feature of fish and a key evolutionary trait underlying fish diversification. Despite frequent and recurrent scale loss throughout fish diversification, comprehensive genome-wide analyses of the genomic signatures associated with scale loss in divergent fish lineages remain scarce. In the current study, we investigated genome-wide signatures, specifically convergent protein-coding gene loss, amino acid substitutions, and cis-regulatory sequence changes, associated with recurrent scale loss in two divergent Cypriniformes lineages based on large-scale genomic, transcriptomic, and epigenetic data.

[Recent advances in the development and application of effervescence-assisted microextraction techniques].

Effervescence-assisted microextraction (EAM) is a novel sample pretreatment method based on the reaction of CO and H donors to generate CO bubbles and promote rapid dispersion of the extractant. During this process, the unique dispersion method increases the contact area between the target molecule and the extraction solvent, and the adsorption/extraction efficiency of the adsorbent/extractant toward the target molecule is also enhanced. The EAM technique is of particular interest due its convenient application, low running costs, reduced solvent consumption, high extraction efficiency, and environmental friendliness.

Ultrafine VO-anchored 3D N-doped carbon nanocomposite with augmented dual-enzyme mimetic activity for evaluating total antioxidant capacity.

Total antioxidant capacity (TAC) can be evaluated by detecting the content of antioxidants, such as ascorbic acid, based on the enzyme-mimetic activity of nanomaterials. Herein, we fabricated a 3D-VO/NC nanocomposite using a self-templating strategy, which achieved ultrafine particles (∼2.5 nm), a porous carbon layer, large specific surface area (152.

Prevalent Introgression Underlies Convergent Evolution in the Diversification of Pungitius Sticklebacks.

New mutations and standing genetic variations contribute significantly to repeated phenotypic evolution in sticklebacks. However, less is known about the role of introgression in this process. We analyzed taxonomically and geographically comprehensive genomic data from Pungitius sticklebacks to decipher the extent of introgression and its consequences for the diversification of this genus.

Colorimetric assay based on NiCoS@N,S-rGO nanozyme for sensitive detection of HO and glucose in serum and urine samples.

Traditional bimetallic sulfide-based nanomaterials often have a small specific surface area (SSA), low dispersion, and poor conductivity, thereby limiting their wide applications in the nanozyme-catalytic field. To address the above issues, we herein integrated NiCoS with N,S-rGO to fabricate a nanocomposite (NiCoS@N,S-rGO), which showed a stronger peroxidase-mimetic activity than its pristine components. The SSA (155.

N, S-co-doped carbon/CoS nanocomposite with dual-enzyme activities for a smartphone-based colorimetric assay of total cholesterol in human serum.

We fabricated a novel N,S-co-doped carbon/CoS nanocomposite (NSC/CoS) using a facile sol-gel approach, which featured a multiporous structure, abundant S vacancies and Co-S nanoparticles filling the carbon-layer pores. When the CoS nanoparticles were anchored onto the surface of N,S-co-doped carbon, a synergistic catalysis action occurred. The NSC/CoS nanocomposites possessed both peroxidase-like and oxidase-mimetic dual-enzyme activities, in which the oxidase-mimetic activity dominated.

Study on the effect of CYP2C19 genetic polymorphism and plasma concentration on clopidogrel resistance.

At present, the mechanism for clopidogrel resistance (CR) is incompletely understood. Here, we aimed to analyze of the association of plasma concentration of clopidogrel active metabolites (CAM) and CYP2C19 genetic polymorphism with CR. We assigned 77 patients to receive CLP at a loading dose of 300mg on day 1, followed by 75mg per day from day 2 to day 6.

Effect of Simulated Geomagnetic Activity on Myocardial Ischemia/Reperfusion Injury in Rats.

Objective: To study the response of myocardial ischemia/reperfusion injury (MI/RI) in rats to simulated geomagnetic activity.

Methods: In a simulated strong geomagnetic outbreak, the MI/RI rat models were radiated, and their area of myocardial infarction, hemodynamic parameters, creatine kinase (CK), lactate dehydrogenase (LDH), melatonin, and troponin I values were measured after a 24-hour intervention.

Results: Our analysis indicates that the concentrations of troponin I in the geomagnetic shielding+operation group were lower than in the radiation+operation group (P<0.

WWOX CNV-67048 Functions as a Risk Factor for Epithelial Ovarian Cancer in Chinese Women by Negatively Interacting with Oral Contraceptive Use.

Copy number variations (CNVs) have attracted increasing evidences to represent their roles as cancer susceptibility regulators. However, little is known about the role of CNV in epithelia ovarian cancer (EOC). Recently, the CNV-67048 of WW domain-containing oxidoreductase (WWOX) was reported to alter cancer risks.

[The cytokine homeostasis was regulated by over-expression of Sirt1 in collagen-induced arthritis mice].

Objective: To investigate the effects of over-expression of Sirt1 on cytokines in the collagen type 2-induced arthritis (CIA) mice.

Methods: The female DBA/1 mouse CIA model was established by subcutaneous injection of collagen type 2. Recombinant adenovirus carrying Sirt1 gene was infected into CIA mice through tail vein injection.

[Application of the vanillin sulfuric acid colorimetry-ultraviolet spectrometry on quality evaluation of Panax notoginseng].

In this study, Panax notoginseng samples were extracted by chloroform, ethanol and water, or by those extracted solution with 5% vanillin sulfuric acid to establish two kinds of UV fingerprint of P. notoginseng which were compared by applying the common and variation peak ratio dual index sequence analysis method and SIMCA software qualitative analysis. The results indicated that the optimization extraction time of P.

[Clinical evaluation of the antioxidant activity of astragalus in women with gestational diabetes].

Objective: To evaluate the antioxidant activity of astragalus and its therapeutic effect on gestational diabetes.

Methods: Eighty-four pregnant women with gestational diabetes were divided into insulin and insulin plus astragalus groups after regular dietary control and insulin treatment to maintain stable blood glucose level. The 43 patients in insulin group received insulin injection, whereas the 41 patients in the other group received treatment with both insulin and astragalus.

Effect of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits.

Objective: To determine the efficacy of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits.

Methods: Twenty-four Japanese white rabbits underwent laminectomy (including the attached ligaments) at L(2 ) and L(5). After laminectomy at L(5), polylactic acid glue was sprayed on the dura and nerve roots and this segment was taken as the experimental group.

[Culture of chondrocytes onto poly-DL-lactide scaffoldings: experimental study].

This experimental study was intended to find the possibility of using PDLLA as scaffolds in tissue engineering and to assess the effect of PDLLA scaffolds with different porosity on chondrocytes. Chondrocytes were cultured when they were seeded onto PDLLA scaffolds with different porosity. Under scanning electron microscope, the scaffolds in hydrophila and adsorptivity to chondrocytes and the function of the cells were observed.

[Experimental study on the polylactic acid glue in preventing epidural adhesion].

Objective: To determine the ability of the polylactic acid glue (PLA-G) in preventing epidural adhesion after laminectomy.

Methods: Each of the 24 randomly selected rats was done laminectomy of L2 and L5. The PLA-G, which would change from liquid to solid when meets with serum, was used in the epidural site of L5 to become a half-solid membrane (the experimental group, EG).

[Effect of absorbable poly-DL-lactide rods on experimental fracture healing].

This study was designed to assess the effect of a home-bred poly-DL-lactide (PDLLA) rods on experimental fracture healing. An operation was performed to create bilateral ulnar and radial fractures of forearm in 15 rabbits. The right sides were fixed with 2 PDLLA (Mv = 34 x 10(4), 1.

[Function of fibula in stability of ankle joint].

Objective: To summarize the function of fibula in stability of ankle joints.

Methods: Recent original articles were extensively reviewed, which were related to the physiological function and biomechanical properties of fibula, the influence of fibular fracture on stability of ankle joints and mechanism of osteoarthritis of ankle joints.

Results: The fibula had the function of weight-bearing; and it was generally agreed that discontinued fibula could lead to intra-articular disorder of ankle joint in children; but there were various viewpoints regarding the influence of fibular fracture on the ankle joint in adults.