Simultaneous Determination of Vitamins A and E and Their Generated Metabolites in Human Serum by LC-MS/MS.

In the context of personalized and precision medicine, simultaneous monitoring of different forms of vitamins A and E and their metabolites could help us better understand the status of vitamins A and E in the body. The aim of this study was to establish a method for simultaneous determination of 13 kinds of vitamins A and E and their metabolites in human serum. Serum samples were directly detected by LC-MS/MS after deproteinization.

Liquid chromatography-tandem mass spectrometry measurement of 26 steroid hormones in human serum and plasma samples.

The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a high-throughput solid-phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes.

Rapid determination of 20 bile acids in human serum by ultra performance liquid chromatography-tandem mass spectrometry.

A method was established for the simultaneous determination of 20 kinds of bile acids in human serum employing ultraperformance liquid chromatography-tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes. The linearity, accuracy and repeatability of the development method were validated with a series of experiments.

Determination of selected polybrominated diphenylethers and polybrominated biphenyl in polymers by ultrasonic-assisted extraction and high-performance liquid chromatography-inductively coupled plasma mass spectrometry.

A new method has been developed for the determination of selected polybrominated diphenylethers (PBDEs) and polybrominated biphenyl (PBB) in four polymers: high-density polyethylene (HDPE), polystyrene (PS), acrylonitrile-butadiene-styrene copolymer (ABS), and polypropylene (PP). PBDEs and PBB in the polymers were extracted with toluene, using ultrasonic-assisted extraction (UAE). The extracts were then determined by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), using external calibration (single-point).